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Plasmid#69236PurposeU6 driven SpCas9 sgRNA expression for TS4 siteDepositorInsertTS4 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-KANK3
Plasmid#69238PurposeU6 driven SpCas9 sgRNA expression for KANK3 siteDepositorInsertKANK3 sgRNA (KANK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-PLXNB2
Plasmid#69240PurposeU6 driven SpCas9 sgRNA expression for PLXNB2 siteDepositorInsertPLXNB2 sgRNA (PLXNB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 PuroR L5-L2
Plasmid#62165PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloningDepositorInsertPuromycin
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-puro/hFOXH1 shRNA6
Plasmid#59310PurposeKnockdown of human FOXH1DepositorInserthFOXH1 shRNA (FOXH1 Human)
UseRNAi and RetroviralTagsExpressionMutationPromoterhU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1-P207S
Plasmid#131726PurposeMammalian Expression of RAF1DepositorInsertRAF1 (RAF1 Human)
UseLentiviralTagsExpressionMammalianMutationP207SPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro LUC (w168-1)
Plasmid#17477Purpose3rd gen lentiviral Luciferase expression vector, CMV promoter, PuroDepositorInsertFirefly Luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-Puro (BRD4)
Plasmid#91796PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-Puro
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Puro (658-5)
Plasmid#17448Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, PuroDepositorHas ServiceCloning Grade DNAInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
lenti-tmpknot-epegRNA-puro backbone
Plasmid#214089PurposeLentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102798PurposeRetrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationH231LPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
Plasmid#202566PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopyDepositorInsertLINE-1
UseTagsmNeonGreen2 on ORF1pExpressionMammalianMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN(CMV-copGFP-Luc2-Puro)
Plasmid#80389PurposeTo make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection markerDepositorInsertcopGFP-T2A-Luc2-F2A-Puromycin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-cOVA-IRES-BFP
Plasmid#135074PurposeExpresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.DepositorInsertcOVA-IRES-BFP (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro mCherry-EGFP-LC3B
Plasmid#22418Purposeretroviral expression of mCherry, EGFP-tagged LC3DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (MAP1LC3B Human)
UseRetroviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1)
Plasmid#17452Purpose3rd gen lentiviral Gateway destination vector, expression, CMV promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-eGFP-Puro
Plasmid#79823PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-KRAB-Puro
Plasmid#99372Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)DepositorInsertdCas9-KRAB-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-3HA-SREBP2-C-Flag
Plasmid#134287PurposeLentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2DepositorInsertSREBP2 (Srebf2 Mouse)
UseLentiviralTags3x HA and FlagExpressionMammalianMutationmouse SREBP2 precursorPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only