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Plasmid#14684DepositorInsertPECAM promoter/enhancer (Pecam1 Mouse)
UseTagsH2B-GFP and PGK-HygroExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP
Plasmid#154247Purposebackbone with PreScission protease-cleavable N-terminal OneStrepFLAG tagDepositorTypeEmpty backboneUseTagsFLAG and Strep-Tag IIExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
YFP-mGCN5-C1
Plasmid#134573PurposeExpresses YFP-GCN5 fusion proteinDepositorInsertGCN5 (Kat2a Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBP-Bba_K822000
Plasmid#74094PurposeL-Lactate inducible promoter from E. coli MG1655DepositorInsertBba_K822000 (L-Lactate promoter - E. coli MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNAAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23100
Plasmid#72963PurposeLevel 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23100 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-GFPIvy-SpHis5
Plasmid#60783PurposeYeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selectionDepositorInsertGFPIvy
UseYeast integratingTagsExpressionMutationS30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…PromoterAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
BII-CPspaH2B
Plasmid#133385PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 9
Plasmid#8476DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…PromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_mCherry_sgEPAS1#1
Plasmid#183122PurposepLentiGuide modified to express mCherry and an sgRNA targeting EPAS1DepositorInsertsgEPAS1-1
UseLentiviralTagsExpressionMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_mCherry_sgEPAS1#2
Plasmid#183123PurposepLentiGuide modified to express mCherry and an sgRNA targeting EPAS1DepositorInsertsgEPAS1-2
UseLentiviralTagsExpressionMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δgag/pol
Plasmid#101343PurposeDeletion from the start of gag until 229 bases before the cPPTDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta GagPolPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-pET-RBS
Plasmid#72981PurposePET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)DepositorInsertpET RBS derived from pET vectors
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH
Plasmid#133391PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP8
Plasmid#135553PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP8
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-dH3-YFP
Plasmid#8694DepositorInsertH3 (His3:CG31613 Fly)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Fyn C3,6S
Plasmid#19979DepositorInsertFyn (FYN Human)
UseTagsExpressionMammalianMutationC3,6SPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-848-952(pL91)
Plasmid#124388PurposeE.Coli produced TET1-848-952 purified by FPLC nickel columnDepositorInsertGB1-His-TET1-848-952 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-C3HTK
Plasmid#133388PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Ptac_BmHps_BmPhi_BsMdh
Plasmid#129103PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicusDepositorInsertBsMDH BmHPS BmPHI
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNC1137
Plasmid#41563DepositorInsertUra3-FUS1-UBIMdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only