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Plasmid#121733PurposepMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO601: pMVP (R4-R3) eCFP open
Plasmid#121735PurposepMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteCFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO401: pMVP (R4-R3) eYFP open
Plasmid#121737PurposepMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteYFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KH305: pMVP (R4-R3) βgal open
Plasmid#121739PurposepMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertβgal (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LF801: pMVP (L1-R5) cTnT promoter
Plasmid#121696PurposepMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertcTnT promoter (Cardiomyocyte specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IH001: pMVP (L5-L4) HA epitope tag
Plasmid#121699PurposepMVP L5-L4 entry plasmid, contains HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsertHA epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IH201: pMVP (L5-L4) 3x HA epitope tag
Plasmid#121700PurposepMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsert3x HA epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX203: pMVP (L5-L4) V5 epitope tag
Plasmid#121705PurposepMVP L5-L4 entry plasmid, contains V5 epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsertV5 epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KU801: pMVP (L5-L4) mCherry
Plasmid#121709PurposepMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest.DepositorInsertmCherry (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KU901: pMVP (L5-L4) eCFP
Plasmid#121711PurposepMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest.DepositorInserteCFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KP401: pMVP (L5-L4) eCFP-P2A
Plasmid#121712PurposepMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.DepositorInserteCFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KV001: pMVP (L5-L4) eYFP
Plasmid#121713PurposepMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.DepositorInserteYFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HB501: pMVP (L1-L4) RIP
Plasmid#121688PurposepMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assemblyDepositorInsertRat Ins1 promoter (RIP; β-cell specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29[dCas9]-1A(lacZ)
Plasmid#126979PurposeLevel 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationCas9 catalytically deadPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClodANL-FGF4
Plasmid#107874PurposeBacterial expression of FGF4-NanoLucDepositorInsertFGF4 (FGF4 Synthetic)
UseLuciferaseTagsNanoLucExpressionBacterialMutationPromoterpro DAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
B. adolescentis mRNA toehold switch sensor
Plasmid#110710PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. adolescentis species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
F. prausnitzii mRNA toehold switch sensor
Plasmid#110716PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertF. prausnitzii species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
C. difficile mRNA toehold switch sensor
Plasmid#110714PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertC. difficile species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
R. hominis mRNA species specific sensor
Plasmid#110713PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertR. hominis species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
E. rectale mRNA toehold switch sensor
Plasmid#110712PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertE. rectale species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only