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Addgene
Showing: 2161 - 2180 of 16055 results
  1. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Availability
    Academic Institutions and Nonprofits only
  2. pGS1122

    Plasmid
    #63790
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker
    Insert
    eSapphire-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eSapphire-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
  3. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
  4. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
  5. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Availability
    Academic Institutions and Nonprofits only
  6. pGS1212

    Plasmid
    #63785
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker
    Insert
    eGFP-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eGFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
  7. pGS1613

    Plasmid
    #63789
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection marker
    Insert
    tDimer2-Strep-tag II followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Availability
    Academic Institutions and Nonprofits only
  8. pUC19-link-mVenus-FLAG:Kan

    Plasmid
    #211830
    Purpose
    Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.
    Depositor
    Insert
    mVenus-FLAG-Kan
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pUC19-link-sfGFP-FLAG:Kan

    Plasmid
    #211833
    Purpose
    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.
    Depositor
    Insert
    sfGFP-FLAG-Kan
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 2161 - 2180 of 16055 results