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Showing: 2161 - 2180 of 4627 results

  2. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Article
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

    Enlarge

  5. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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  6. ATX3-77Q

    Plasmid
    #184249
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Codon optimization for protein expression in BL2…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    184249-plasmid-map-sequence-id-370367

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  7. ATX3-77Q-R388G

    Plasmid
    #184251
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    184251-plasmid-map-sequence-id-409179

    Enlarge

  9. pD603 H77 E1E2

    Plasmid
    #86983
    Purpose
    Mammalian expression vector encoding codon optimised Heptitis C virus H77 glycoproteins E1E2
    Depositor
    Joe Grove
    Insert
    HCV E1E2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    86983-plasmid-map-sequence-id-195168

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  10. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid
    #132524
    Purpose
    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Insert
    DYNC2H1 (DYNC2H1 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, Precission cleavag…
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    132524-plasmid-map-sequence-id-261476

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  11. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Availability
    Academic Institutions and Nonprofits only
    205412-plasmid-map-sequence-id-416556

    Enlarge

  12. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV1)

    Viral Prep
    #87306-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Article
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only

  13. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV2)

    Viral Prep
    #87306-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Article
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only

  14. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV5)

    Viral Prep
    #87306-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Article
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only

  15. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV8)

    Viral Prep
    #87306-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Article
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only

  16. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV9)

    Viral Prep
    #87306-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Article
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Tags
    None
    Availability
    Academic Institutions and Nonprofits only

  17. pTR-UF11 (AAV.A01.T)

    Viral Prep
    #157970-AAV.A01.T
    Purpose
    Ready-to-use AAV2 in TMN200 particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter
    Depositor
    Sergei Zolotukhin
    Article
    Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10
    Promoter
    chimeric CMV/Chicken Beta actin (CBA)
    Tags
    GFP
    Availability
    Academic Institutions and Nonprofits only

  18. pTR-UF11 (AAV.A02.T)

    Viral Prep
    #157970-AAV.A02.T
    Purpose
    Ready-to-use AAV2 in PBS particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter
    Depositor
    Sergei Zolotukhin
    Article
    Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10
    Promoter
    chimeric CMV/Chicken Beta actin (CBA)
    Tags
    GFP
    Availability
    Academic Institutions and Nonprofits only

  19. pTR-UF11 (AAV.A03.T)

    Viral Prep
    #157970-AAV.A03.T
    Purpose
    Ready-to-use AAV2 in BSS particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter
    Depositor
    Sergei Zolotukhin
    Article
    Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10
    Promoter
    chimeric CMV/Chicken Beta actin (CBA)
    Tags
    GFP
    Availability
    Academic Institutions and Nonprofits only

  20. pTR-UF11 (AAV.A04.T)

    Viral Prep
    #157970-AAV.A04.T
    Purpose
    Ready-to-use AAV2 (Y444F) in TMN200 particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter
    Depositor
    Sergei Zolotukhin
    Article
    Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10
    Promoter
    chimeric CMV/Chicken Beta actin (CBA)
    Tags
    GFP
    Availability
    Academic Institutions and Nonprofits only
Showing: 2161 - 2180 of 4627 results