Addgene

We narrowed to 4,770 results for: codon optimized

Showing: 2161 - 2180 of 4770 results

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pYPQ151

Plasmid

#69302

Purpose

Gateway entry vector with pcoCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9D10A (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Expression

Mutation

D10A mutation; NLS fusion

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRS403red-luxTA2-4F

Plasmid

#166030

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405red-phlTA2-1E

Plasmid

#166029

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSZT025

Plasmid

#140033

Purpose

KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

cLac143

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGK415-luxTA2-4F

Plasmid

#165971

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET32-sLatherin

Plasmid

#61685

Purpose

bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

Depositor

Brian Smith

Article

Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

Insert

Latherin (LATH horse)

Use

Tags

HIS and Trx-Tag thioredoxin protein

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

T7

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-T3-hCasD10A-pA

Plasmid

#51638

Purpose

Expresses Cas9-nickase in mammalian cells and zygotes.

Depositor

Wataru Fujii

Article

Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

Insert

Codon-optimized Cas9 D10A

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
Pprm1-luciferase; his-3

Plasmid

#137728

Purpose

Communication-activated luciferase in Neurospora crassa

Depositor

Louise Glass

Article

Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

Inserts

Luciferase
Pprm-1 Promoter

Use

Luciferase; Neurospora crassa expression

Tags

Expression

Mutation

Codon-optimized for N. crassa

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-NLS-HA-B3

Plasmid

#51270

Purpose

Expression of the site-specific recombinase B3 (mouse codon-optimized)

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-B3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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PARP15-c021

Plasmid

#173102

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP15 (PARP15 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

G287-N470, codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-coEPO-lox2272

Plasmid

#154828

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

coEPO

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

100RPU.2

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p33Fn

Plasmid

#158155

Purpose

Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Rice codon optimized

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Tags

Expression

Mutation

D10A mutation; NLS fusion

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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p32Fn

Plasmid

#158153

Purpose

Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Rice codon optimized

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx2(yeast)

Plasmid

#65005

Purpose

Yeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

GRX2

Use

Tags

roGFP2

Expression

Yeast

Mutation

Promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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p1148-pCSDest-AcrIIA4-mCherry

Plasmid

#126204

Purpose

Mammalian expression of AcrIIA4 fused to mCherry

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIA4

Use

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCSN074

Plasmid

#166731

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV rev

Plasmid

#101339

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized rev

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGK415-phlTA2-1E

Plasmid

#165970

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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