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Showing: 2141 - 2160 of 17469 results

  1. pGBW-m4134897

    Plasmid
    #151948
    Purpose
    Mammalian expression plasmid for Human coronavirus HKU1 nucleocapsid
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    Human coronavirus HKU1 nucleocapsid phosphoprotein (N Human coronavirus HKU1)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151948-plasmid-map-sequence-id-306273

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  2. pBABE-puro SHP1 deltaP

    Plasmid
    #8576
    Depositor
    Ben Neel
    Insert
    SHP1 (PTPN6 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    deltaP (deletion of phosphatase domain, amino aci…
    Promoter
    Available since
    June 17, 2005
    Availability
    Academic Institutions and Nonprofits only

  3. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  4. LZRS-Mek1-wt1 (wt1)

    Plasmid
    #21196
    Depositor
    Paul Khavari
    Insert
    Mek1 (MAP2K1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  5. 1070 pBabe puroL PTEN 360-364A A4

    Plasmid
    #10943
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    360-364A (SSSTS to AAAAA); A4: S380A, T382A, T383…
    Promoter
    Available since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  7. pLenti puro HA-TUBA1B

    Plasmid
    #227587
    Purpose
    Lentiviral plasmid expressing human TUBA1B protein
    Depositor
    Melina Fan
    Insert
    TUBA1B (TUBA1B Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    227587-plasmid-map-sequence-id-449980

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  8. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

    Plasmid
    #217970
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    217970-plasmid-map-sequence-id-436105

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  9. LV-sgRNA-mCherry-Puro sgPIDD

    Plasmid
    #211528
    Purpose
    Deletes PIDD
    Depositor
    Richard Possemato
    Insert
    sgPIDD
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    211528-plasmid-map-sequence-id-421240

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  10. pLZRS-mVenus-TrkC

    Plasmid
    #182948
    Purpose
    To express the rat TrkC fused in the N-terminus with mVenus
    Depositor
    Pantelis Tsoulfas
    Insert
    mVenus-TrkC (Ntrk3 Synthetic, Rat)
    Use
    Retroviral and Synthetic Biology
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182948-plasmid-map-sequence-id-360203

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  11. LentiCRISPR_Hs_MBD3_ex2

    Plasmid
    #62623
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. LentiCRISPR_Hs_MBD3_ex3

    Plasmid
    #62624
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  16. LCV2_EGFP

    Plasmid
    #155098
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155098-plasmid-map-sequence-id-307983

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  17. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid
    #132389
    Purpose
    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauWT 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132389-plasmid-map-sequence-id-270333

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  19. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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Showing: 2141 - 2160 of 17469 results