-
Plasmid#16155DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationS400APromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II-YPet(short)
Plasmid#118714PurposeThe human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control.DepositorInsertDesmoplakin II-YPet(short) (DSP Human)
UseRetroviralTagsYPet(short)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-msGC-NT13
Plasmid#75087Purposeexpresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and betaDepositorInsertsfragment of alfa subunit of sGC
fragment of beta subunit of sGC
UseTagsHis-tagExpressionBacterialMutationfragment 1-380 of the beta subunit and fragment 4…PromoterAvailable sinceMay 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160
Plasmid#74607PurposeExpresses Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFS_0385_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1
Plasmid#116997PurposeExpresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertRivularia sp. PCC 7116 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH-C234S
Plasmid#107724PurposeExpression of PHGDH C234S in bacteriaDepositorInsertPHGDH (PHGDH Human)
UseTagsExpressionBacterialMutationC234SPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-LpxA
Plasmid#12684DepositorInsertUDP-N-acetylglucosamine acyltransferase (HP1375 Helicobacter pylori)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-CylA
Plasmid#48198PurposeExpresses CylA (fatty acyl-AMP ligase) in E. coliDepositorInsertCylA
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
LHP2691 - pET30-Ub-K63A
Plasmid#32179DepositorUseTagsHISExpressionBacterialMutationK63APromoterT7Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
LHP2690 - pET30-Ub-K48A
Plasmid#32178DepositorUseTagsHISExpressionBacterialMutationK48APromoterT7Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET26-CNTN149S/F366A
Plasmid#100165Purposerecombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target proteinDepositorInsertConcentrative nucleoside transporter
UseTagsMBP-HisExpressionBacterialMutationN149S, F366APromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Dlux
Plasmid#47657PurposePesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-D
UseSynthetic BiologyTagsExpressionBacterialMutationAdded an additional esa box upstream of -35 site …PromoterPesaR-DAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-H74A-PvuRts1I
Plasmid#86894Purposeexpress H74A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationW205APromoterT7Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hTIG3-1-164
Plasmid#100724PurposeFull-length human TIG3 in E.coli expression vectorDepositorInsertRARRES3 (PLAAT4 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Dog 1-77)
Plasmid#62060PurposeExpression of Dog p53 (residues 1-77) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-77PromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a pro-RegIIIg
Plasmid#64934Purposebacterial expression of full length mouse RegIIIgammaDepositorInsertRegIIIg (Reg3g Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-5F5-mCherry
Plasmid#73422PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.DepositorInsertPromoter 5F5 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP5F5 (orthogonal T7-lac variant)Available sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 L171C
Plasmid#66177PurposeExpresses the L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationL171CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C
Plasmid#66140PurposeExpresses the G108C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hTIG3-1-132
Plasmid#100720PurposeExpression of N-terminal region of human TIG3 in E.coliDepositorInsertRARRES3 (PLAAT4 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only