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Showing: 2141 - 2160 of 2425 results

  4. TDP-43 mRuby K84ONBK

    Plasmid
    #216226
    Purpose
    TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.
    Depositor
    Jennifer Lee
    Article
    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do
    Insert
    Transactive response DNA binding protein of 43 kDa (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lysine 84 mutated to a TAG stop codon
    Promoter
    CMV Promoter
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216226-plasmid-map-sequence-id-431056

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  6. pEGFP_∆DD-talin1 (1-2493)

    Plasmid
    #166124
    Purpose
    For expression of mouse talin-1 (residues 1-2493) containing C-terminal deletion of dimerization domain (∆DD) in mammalian cells. Contains C-terminal EGFP tag.
    Depositor
    Vesa Hytönen
    Article
    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.
    Insert
    ∆DD-Talin-1 (Tln1 Mouse)
    Use
    Cmv promoter
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    residues 1-2493, deletion of dimerization domain …
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166124-plasmid-map-sequence-id-330288

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  7. sgFFL_d23

    Plasmid
    #59892
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd and fourth seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59892-plasmid-map-sequence-id-108249

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  8. sgFFL_d3

    Plasmid
    #59891
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59891-plasmid-map-sequence-id-108247

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  10. PB513B-1/TRE-hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ-EF1α-Puro

    Plasmid
    #199550
    Purpose
    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoter
    Depositor
    Masamichi Kamihira
    Article
    Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33
    Insert
    hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ (HNF1A, FOXA2, CEBPB, CEBPA, CEBPG Human)
    Use
    Piggybac transposon vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE+CMVmin promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    199550-plasmid-map-sequence-id-405449

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  12. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

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  13. pRosa26-GT

    Plasmid
    #40025
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    beta-globin intron
    Neo
    tdT-3Myc
    diphteria toxin A
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta globin promoter and CMV enhance…
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    40025-plasmid-map-sequence-id-210933

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  18. Cx30-msfGFP

    Plasmid
    #69019
    Purpose
    Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx30 (GJB6 Human)
    Use
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    c
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69019-plasmid-map-sequence-id-127981

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  19. pCSF107mT-GATEWAY-3'-3HA

    Plasmid
    #67616
    Purpose
    A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology
    Depositor
    Todd Stukenberg
    Article
    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi
    Type
    Empty backbone
    Use
    In vitro transcription or translation off of an s…
    Tags
    three inframe 3' HA tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and SP6
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2141 - 2160 of 2425 results