-
Plasmid#105137PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mEGFP
UseTags3nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE E171Q GFP
Plasmid#32128DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, E171QPromoterAvailable sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nup88 (551-741)
Plasmid#64252PurposeMammalian expression of C-terminal domain of Nup88DepositorInsertNup88 (NUP88 Human)
UseTagshis and mycExpressionMammalianMutationC-terminal domain (551-741)PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p201N 1514
Plasmid#47025PurposeContains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencingDepositorInsertsgma-miR1514 recognition sequence
nptII selectable marker
UseRNAiTagsExpressionMutationPromoterGmUbi and StUbi-3Available sinceAug. 30, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTrc-clbS_Ealbertii
Plasmid#223360PurposeEncoding ClbS from Escherichia albertii CB9786DepositorInsertEncoding ClbS from Escherichia albertii CB9786 (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Bifido
Plasmid#223359PurposeEncoding ClbS from Bifidobacterium longum subsp. infantisDepositorInsertEncoding ClbS from Bifidobacterium longum subsp. infantis (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Samsonia
Plasmid#223357PurposeEncoding ClbS from Samsonia erythrinae DSM 16730DepositorInsertEncoding ClbS from Samsonia erythrinae DSM 16730 (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Gibsiella
Plasmid#223356PurposeEncoding ClbS from Gibbsiella quercinecans DSM 25889DepositorInsertEncoding ClbS from Gibbsiella quercinecans DSM 25889 (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Snod
Plasmid#223355PurposeEncoding ClbS from Snodgrassella alvi wkB2DepositorInsertEncoding ClbS from Snodgrassella alvi wkB2 (Codon optimized for E coli)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc-clbS_Dickeya
Plasmid#223351PurposeEncoding ClbS from Dickeya dadantiiDepositorInsertEncoding ClbS from Dickeya dadantii (Codon optimized for E coli) (CO076_RS11420 Dickeya dadantii)
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-MiniR
Plasmid#208328PurposeMiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.DepositorInsertH2B-MiniR
UseTagsMiniRExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-TinyERRE
Plasmid#208324PurposeC-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-TinyERRE
UseTagsTinyERREExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-PuRRRE
Plasmid#208329PurposePuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.DepositorInsertTOMM20-PuRRRE
UseTagsPuRRREExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-PunyEEER
Plasmid#208326PurposeC-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-PunyEEER
UseTagsPunyEEERExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK809
Plasmid#196406PurposeStrong expression of firefly and nanoluc luciferases codon-optimized for S. rosettaDepositorInsertSro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL1P6 ZmUbiP:GRF-GIF:NosT
Plasmid#198049PurposeMoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyTagsExpressionMutationPromoterZea mays (maize) ubiquitin promoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENmRFPCNAL2 pc1054
Plasmid#167567PurposeThe fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis
Plasmid#176573PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q W330Y)-6xHis
Plasmid#176577PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3b
Plasmid#166054Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertOrbicella faveolata caspase-3b
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only