-
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiRTi
Plasmid#214068PurposeFluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRTX_ctrl
Plasmid#214069PurposeFluorescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRT
Plasmid#214067PurposeFluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRT_RTctrl
Plasmid#214070PurposeFluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL11015
Plasmid#117499PurposeFAST-Red selectable marker Golden Gate Level 1 Position 1DepositorInsertBpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
UseTagsRFPExpressionPlantMutationPromoterAtOLE1Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-A
Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpltetO1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL50004
Plasmid#50316DepositorInsertCDS, mCherry variant of RFP (Discosoma sp.)
UseTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
BASIC_7_J23101-RBS34-mCherry-B0015
Plasmid#68141Purposestorage vector for BASIC part: RFP expression cassetteDepositorInsertJ23101-RBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL80007
Plasmid#50323DepositorInsertCDS, mCherry variant of RFP (Discosoma sp.)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL30003
Plasmid#50304DepositorInsertCDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
UseTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL80004
Plasmid#50325DepositorInsertCDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD010
Plasmid#116851PurposeExpresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010DepositorInsertsAraC
alpha-HL
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS012
Plasmid#65465PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherryDepositorInsertNtag-mCherry
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
JC101
Plasmid#104952PurposeMethanol induing RFP, estrogen inducing GFP, and IPTG inducing CFPDepositorInsertGFP, mCherry, and CFP
UseTagsExpressionMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-P38-che-ssrAec3
Plasmid#27059DepositorInsertP#38-mcherry rfp-ssrAec
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICH49477
Plasmid#50324DepositorInsertCDS, DsRed variant of RFP (Discosoma sp.)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only