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Plasmid#188088PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (723-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (828-839)
Plasmid#188090PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (828-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK880-pCAG-DmrC-NLS-3xFLAG-VP64
Plasmid#136912PurposeDmrC with VP64 fused to it's C-terminus; Mammalian expression vectorDepositorInsertpCAG-DmrC-NLS-3xFLAG-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterChicken Beta ActinAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector
Plasmid#182346PurposePacI-flanked CAG-LSL expression cassette with a FNF for positive selectionDepositorInserttdTomato
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP-squirrel-monkey-CHMP3(155)
Plasmid#154183Purposeexpresses squirrel monkey CHMP3(155) in mammalian cellsDepositorInsertCHMP3(155)
UseTagsFLAG, Strep-Tag II, and preScission siteExpressionMammalianMutationC-terminal truncation of CHMP3 at amino acid 155PromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E
Plasmid#125797PurposeHistone H2B-superfolder GFP and tdTomato fused by unskippable linkerDepositorInsertH2BsfGFP-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA
Plasmid#108820PurposeExpresses aa252 to C-terminus of Cre recombinase fused to pMag CIDDepositorInsertCre recombinase fragment aa252 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA
Plasmid#108822PurposeExpresses aa257 to C-terminus of Cre recombinase fused to pMag CIDDepositorInsertCre recombinase fragment aa257 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA
Plasmid#108800PurposeExpresses aa397 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA
Plasmid#108804PurposeExpresses aa572 to C-terminus of PhiC31 integrase fused to PYL CIDDepositorInsertPhiC31 integrase fragment aa572 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-sfGFP-tdTomato T2A Q XE
Plasmid#113852PurposeSuperfolder GFP and tdTomato linked by T2A (Q linker)DepositorInsertsfGFP-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A V XE
Plasmid#113843PurposeHistone H2B-superfolder GFP and tdTomato linked by T2A (V linker)DepositorInsertH2B-sfGFP-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A Q XE
Plasmid#113842PurposeHistone H2B-superfolder GFP and tdTomato linked by T2A (Q linker)DepositorInsertH2B-sfGFP-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpA
Plasmid#87576PurposePV3 for 2-input, 4-output decoder circuitDepositorInsertiRFP720
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpA
Plasmid#87582PurposePV3 for 3-input, 2-output Full AdderDepositorInsertEGFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
Plasmid#107270PurposeExpresses Cas9 upon induction with doxicycline.DepositorInsertshSpCas9
rtTA
bGH polyA-SV40polyA
UseTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA
Plasmid#183775PurposeAAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertNLS-GFP
UseAAVTagsExpressionMutationNAPromoterCAGAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA
Plasmid#108729PurposeExpresses N-terminus to aa270 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa270
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA
Plasmid#108730PurposeExpresses aa271 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]
Plasmid#195488PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.DepositorInsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCAGAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only