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Plasmid#221477PurposeGateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR knockout HRD
Plasmid#207579PurposeHomologous recombination donor replacing TR with a puro cassette.DepositorInsertpuro resistance cassette flanked by TR locus sequences excluding TR
UseTagsNoneExpressionMammalianMutationPromoterSV40Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-Tn5-chlorR
Plasmid#199994PurposeLanding Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.DepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-pR-himar-apraR
Plasmid#199996PurposeLanding Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donorDepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NeuA.pJL1
Plasmid#199115PurposeIn vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)DepositorInsertN-acylneuraminate cytodiylyltransfease (NeuA)
UseTagsStrep-tagExpressionBacterialMutationPromoterT7Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.539
Plasmid#184958PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
UseTagsExpressionBacterialMutationfabH donor G954APromoterT7/lacAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.543
Plasmid#184960PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
UseTagsExpressionBacterialMutationmurF donor G1359APromoterT7/lacAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.545
Plasmid#184962PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
UseTagsExpressionBacterialMutationpriB donor G1069APromoterT7/lacAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL237
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC4 Grin1-smFP-V5 KI
Plasmid#183439PurposeFlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)DepositorInsertgRNA and smFP V5 donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-3xHA-Syn-smFP-flag
Plasmid#182563PurposeFor mouse Nav1.6 knockin with 3xHA at the C-terminalDepositorInsertsmouse Scn8a gRNA and 3xHA donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-HaloTag-V5-EF1-sfGFP
Plasmid#182565PurposeFor mouse Nav1.6 knockin with HaloTag-V5 at the C-terminalDepositorInsertsmouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn8a-3xV5-EF1-smFP-flag
Plasmid#182562PurposeFor mouse Nav1.6 knockin with 3xV5 at the C-terminalDepositorInsertsmouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-pleCopyCatcher
Plasmid#174061PurposeCopyCatcher insertion donor for the pale locusDepositorInsertPale (Pale Synthetic, Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Hsa-Serum Albumin SP
Plasmid#153496PurposeHuman Serum Albumin Signal Peptide Donor PlasmidDepositorInsertSerum Albumin SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Killer Protein SP
Plasmid#153498PurposeS. cerevisiae Killer Signal Peptide Donor PlasmidDepositorInsertKiller Protein SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Cfo-CPO SP
Plasmid#153500PurposeCaldariomyces (Leptoxyphium) fumago Chloroperoxidase Signal Peptide Donor PlasmidDepositorInsertChloroperoxidase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Cgl-UPO SP
Plasmid#153501PurposeChaetomium globosum Unspecific Peroxygenase Signal Peptide Donor PlasmidDepositorInsertUnspecific peroxygenase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Cci-UPO SP
Plasmid#153502PurposeCoprinopsis cinerea Unspecific Peroxygenase Signal Peptide Donor PlasmidDepositorInsertUnspecific peroxygenase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only