Addgene

We narrowed to 4,627 results for: codon optimized

Showing: 2121 - 2140 of 4627 results

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pPP444

Plasmid

#158802

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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p32Fn

Plasmid

#158153

Purpose

Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Rice codon optimized

Promoter

Availability

Academic Institutions and Nonprofits only

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p33Fn

Plasmid

#158155

Purpose

Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Rice codon optimized

Promoter

Availability

Academic Institutions and Nonprofits only

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pCSN069

Plasmid

#166728

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-Mxi1

Use

CRISPR

Tags

Mxi1

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Availability

Academic Institutions and Nonprofits only

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pCSN074

Plasmid

#166731

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Availability

Academic Institutions and Nonprofits only

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pCSN075

Plasmid

#166732

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Availability

Academic Institutions and Nonprofits only

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BlatCas9

Plasmid

#163792

Purpose

The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

Depositor

Yongming Wang

Article

Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pJJF152_Phsp_Cas9

Plasmid

#163862

Purpose

heat shock-induced expression of Cas9 in C. elegans

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

Cas9, C. elegans codon optimized with intron and SV40_NLS

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Phsp-16.48

Availability

Academic Institutions and Nonprofits only

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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCRISPR-PE-bacteria

Plasmid

#172715

Purpose

Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

Cas9n-linker-M-MLV2

Use

Tags

Expression

Bacterial

Mutation

H840A of Cas9

Promoter

Availability

Academic Institutions and Nonprofits only

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PARP15-c020

Plasmid

#173101

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP15 (PARP15 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

N78-S267, codon optimized

Promoter

Availability

Academic Institutions and Nonprofits only

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PARP15-c021

Plasmid

#173102

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP15 (PARP15 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

G287-N470, codon optimized

Promoter

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP wild type

Plasmid

#174075

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Use

Tags

His-6

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP-150TAG

Plasmid

#174076

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Use

Tags

His-6

Expression

Bacterial

Mutation

N150TAG

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pGK415-phlTA2-1E

Plasmid

#165970

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Availability

Academic Institutions and Nonprofits only

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pGK415-luxTA2-4F

Plasmid

#165971

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Availability

Academic Institutions and Nonprofits only

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pRS405red-phlTA2-1E

Plasmid

#166029

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Availability

Academic Institutions and Nonprofits only

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pRS403red-luxTA2-4F

Plasmid

#166030

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Availability

Academic Institutions and Nonprofits only

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pVoPo-mSc

Plasmid

#187842

Purpose

Constitutive mScarlet-I-expression vector for Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Insert

mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTU424

Plasmid

#178983

Purpose

pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

TPR-CHAT

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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