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Showing: 2121 - 2140 of 4770 results

  2. pCRISPR-S12

    Plasmid
    #84031
    Purpose
    To episomally express codon optimized Cas9 and chimeric guide RNA
    Depositor
    Sen Wu
    Article
    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:
    Inserts
    hSpCas9
    eGFP
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and CMV (downstream of F2A self-cleaving pept…
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    84031-plasmid-map-sequence-id-192362

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  4. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Article
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

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  8. pSBY3_ligD

    Plasmid
    #104624
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104624-plasmid-map-sequence-id-201663

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  10. pDHt/sk-PE

    Plasmid
    #92126
    Purpose
    Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Article
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Insert
    Cas9-eGFP
    Use
    CRISPR; Trichoderma reesei expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Ppdc
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    92126-plasmid-map-sequence-id-195058

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  11. pCEP4-SERT-myc

    Plasmid
    #107456
    Purpose
    Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107456-plasmid-map-sequence-id-205545

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  12. lentiCRISPR - AAVS1 sgRNA

    Plasmid
    #70661
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against AAVS1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pDD284

    Plasmid
    #66825
    Purpose
    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2121 - 2140 of 4770 results