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Showing: 2101 - 2120 of 20525 results

  1. pADH147

    Plasmid
    #90991
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    90991-plasmid-map-sequence-id-180524

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  7. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    102560-plasmid-map-sequence-id-198595

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  13. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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Showing: 2101 - 2120 of 20525 results