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Plasmid#140453PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, phenylalanine 588 to val…PromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-Ef1A-Myc-POLB(PAMmut)
Plasmid#176150PurposeN-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsMYCExpressionMammalianMutationMutation in the PAM site used by POLB gRNA1PromoterEF1AAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB
Plasmid#166410Purposeexpress BirA-hPOT1 ΔOB in mammalian cellsDepositorInsertPOT1 ΔOB (POT1 Human)
UseTagsMYC-flag-BirAExpressionMammalianMutationΔOBPromotercmvAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DED/AAA 535-888 USP7
Plasmid#131255PurposeMammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
IZ702: pMVP (L3-L2) myc epitope tag + WPRE
Plasmid#121760PurposepMVP L3-L2 entry plasmid, contains myc epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsertmyc epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti C-MYC-DDK Puro PNRC1-W300A
Plasmid#123301PurposeMammalian expression of PNRC1-W300A mutant C-MYC-FLAGDepositorInsertPNRC1 (PNRC1 Human)
UseLentiviralTagsMYC-FLAGExpressionMammalianMutationW300APromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA
Plasmid#110656PurposeLentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5cmyc2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3- myc NES-mCE(K294A) NLS
Plasmid#82467PurposeExpresses cytoplasmic restricted form of myc tagged mRNA capping enzymeDepositorInsertNLS deleted from mouse Capping Enzyme (Rngtt Mouse)
UseTagsExpressionMammalianMutationdeletion of KRKY from 573-657PromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His
Plasmid#99743PurposeExpresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.DepositorInsertStx3 (STX3 Human)
UseTagsmyc-myc-hisExpressionMammalianMutationChanged both L165 and E166 to AlaninePromoterCMVAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP
Plasmid#99730Purposeoverexpression of Tmeff2 and mCherryDepositorInsertTmeff2 (Tmeff2 Mouse)
UseTags2xMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 2CS_new (C278S, C329S)
Plasmid#84659Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Laforin Myc 2KA (K87A, K320A)
Plasmid#84660Purposemammalian expression and/or retrovirus preparationDepositorArticleInsertLaforin (EPM2A Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoter5'LTRAvailable sinceNov. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS S4144D-Myc/His
Plasmid#83459PurposeHuman PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)DepositorInsertPolycystin-1 (PKD1 Human)
UseTags6xHis and MycExpressionMammalianMutationAmino-acids 4107-4303, S4144DPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/ TO myc-cCE-bio (cCE-bio)
Plasmid#82473PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive formDepositorInsertMyc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
UseTagsTEV-Biotin and mycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP (N162D)_3xFlag-Myc-CMV26
Plasmid#73578PurposeExpress mouse OC-STAMP ((N162D) in mammalian cellsDepositorInsertOC-STAMP (Ocstamp Mouse)
UseTags3x Flag and c-Myc tagExpressionMammalianMutationchanged asparagin (N) 162 to Aspartic Acid (D)PromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycLAM
Plasmid#71807PurposeGenerating transgenic flies for 4-hydroxytamoxifen-inducible DamIDDepositorInsertFL.hsp70P-Dam[4-HT-intein@L127C]-Myc-LAM
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC
Plasmid#71808PurposeGenerating transgenic flies for 4-hydroxytamoxifen-inducible DamIDDepositorInsertFL.hsp70P-Dam[4-HT-intein@L127C]-Myc-PC
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-Tudor-attB
Plasmid#44210DepositorInsertQin-Tudor-domain (qin Fly)
UseFly transformationTagsFlag and MycExpressionMutationlacks E3 domain on N-term (del aa1-274)PromoterAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only