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Plasmid#107461PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A331T conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationA331TPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTA169D-myc
Plasmid#107460PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A169D conferring gain of function phenotype, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationA169DPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2
Plasmid#79380PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT1
Plasmid#67116PurposeStandard for cell-free expression benchmarking.DepositorInsertSTAT1 (STAT1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C/P186C
Plasmid#66174PurposeExpresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108C, P186CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-miR-28
Plasmid#35686DepositorInserthsa-miR-28 (MIR28 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pEBB-GFP-hBEN-deltaSS
Plasmid#22159DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGFPExpressionMammalianMutationDeletion of amino acids 897-908PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEBG-hBEN-deltaNLS3
Plasmid#22161DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGST and HisExpressionMammalianMutationDeletion of amino acids 883-889PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEBG-hBEN-deltaSS
Plasmid#22162DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGST and HisExpressionMammalianMutationDeletion of amino acids 897-908PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1
Plasmid#79379PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4
Plasmid#79382PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1 CARD8-TEV-GFP-FLAG
Plasmid#169991PurposeMammalian purification construct for CARD8DepositorInsertCARD8 (CARD8 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-8
Plasmid#208786PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorInsertCaspase 8 (CASP8 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E2
Plasmid#164719PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-WT-STAT1
Plasmid#71454PurposeLentiviral expression of WT-STAT1DepositorInsertSTAT1 (STAT1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycIGF2BP3
Plasmid#19879DepositorInsertInsulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-METTL14
Plasmid#53740PurposeFor expression of METTL14 (methyltransferase-like 14) in human cellsDepositorInsertMethyltransferase-like 14 (METTL14 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only