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Plasmid#65695PurposeExpresses Golgi-ONL in mammalian cellsDepositorInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSALECTNK-TEM1(S70A,D179G)/csTEM1
Plasmid#81163PurposepSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1DepositorInsertTEM-1 beta-lactamase
UseTagsCodon swapped TEM-1 from H26 to W290 and His6xExpressionBacterialMutationDeleted the sec transport tag. Plasmid encodes H2…Promoterlac promoterAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
px330-UFSP2 sgRNA1
Plasmid#134638Purposecontains sgRNA targeting human UFSP2 for gene knockoutDepositorInsertUFSP2 sgRNA1 (UFSP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_ITPKA
Plasmid#65688PurposeExpresses ITPKA-CNL in mammalian cellsDepositorInsertinositol triphosphate 3-kinase A
UseLuciferaseTagsCNLExpressionMammalianMutationcontains residues 9-52PromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_RhoB
Plasmid#65697PurposeExpresses CNL-RhoB in mammalian cellsDepositorInsertRhoB (RHOB Human)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
416-Met25 Kar2 printor HDEL
Plasmid#32129DepositorInsertPrintor (KLHL14 Human)
UseTagsHDEL and Kar2ExpressionYeastMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMTH PKC epsilon
Plasmid#8413DepositorInsertMouse Protein Kinase C epsilon (Prkce Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_zyxin
Plasmid#65681PurposeExpresses Zyxin-YNL in mammalian cellsDepositorInsertzyxin (Zyx Mouse)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTH PKC eta
Plasmid#8415DepositorInsertMouse Protein Kinase C eta (Prkch Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_3xNLS
Plasmid#65673PurposeExpresses NLS-CNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_3xNLS
Plasmid#65672PurposeExpresses NLS-YNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
SAC1delta452-587-FKBP-mCherry
Plasmid#108131PurposeRecruitable inactive SAC1 (linker deletion)DepositorInsertSACM1L(1-451):FKBP1A(3-108):mCherry (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-451)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_Mito
Plasmid#65669PurposeExpresses Mito-YNL in mammalian cellsDepositorInsertcytochrome c oxidase subunit VIII
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLTR PKC epsilon
Plasmid#8420DepositorInsertMouse Protein Kinase C epsilon (Prkce Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_zyxin
Plasmid#65683PurposeExpresses Zyxin-ONL in mammalian cellsDepositorInsertzyxin (Zyx Mouse)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRa37_3390
Plasmid#49697DepositorInsertECF37_3390
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_3xNLS
Plasmid#65674PurposeExpresses NLS-ONL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only