-
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
UseTags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA1-CH-mneo
Plasmid#51288Purposecontains the fully codon optimized ORF1 and ORF2 of the human L1RPDepositorInsertL1 (L1RE1 Human)
UseTagsmneoI indicator cassette containing an inverted n…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD268
Plasmid#132523PurposemNG^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mNGExpressionWormMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_9
Plasmid#60580Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_9
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (PX165)
Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9
Plasmid#61433PurposeBinary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequenceDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoterPcUBI4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
Plasmid#128424PurposeHuman Codon Optimized dCas9-DNMT3A3LDepositorInsertdCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterT7 PromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE2D3
Plasmid#186805PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.DepositorInsertHis-Tev-UbE2D3 (UBE2D3 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1-Lyn-mCitrine-EEVEE-LifeAct
Plasmid#155226PurposeAlternate MPAct using Lyn/LifeAct instead of CaaX/F-tractinDepositorInsertLyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE1
Plasmid#186804PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.DepositorInsertHis-Tev-UbE1 (UBA1 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-NLS-I-PpoI
Plasmid#46963PurposeFor constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.DepositorInsertNLS-I-PpoI
UseTagsHAExpressionMammalianMutationPromoterCMV-TETAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pH-tdGFP
Plasmid#74322PurposepH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiaeDepositorInsertpH-tdGFP
UseYeast taggingTagsnoneExpressionYeastMutationN149Y, Q204HPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_EEE_E1E2
Plasmid#207272PurposeMammalian expression of Eastern equine encephalitis virus E1E2 proteinDepositorInsertEEE_E1E2 (EEEVgp3 Eastern equine encephalitis virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN7HELIX
Plasmid#190662PurposeExpresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
UseTagsExpressionMammalianMutationnAniI = K227M, F80K, L232KPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM134
Plasmid#127215PurposeBeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDSDepositorInsertLuc, IPT, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9
Plasmid#110183PurposeExpression of the dCas9 gene in StreptomycesDepositorInsertdCas9
UseCRISPR; ITagsExpressionBacterialMutationD10A, H840APromoterermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCAS-pA
Plasmid#48625PurposeExpresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized Cas9
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMM135
Plasmid#127216PurposeBeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDSDepositorInsertLuc, WUS2, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-his-LbCpf1-EC
Plasmid#79008PurposeBacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)DepositorInsertLbCpf1
UseTagsMBP-6xHis-NLSExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only