Addgene

We narrowed to 2,263 results for: 828

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pEMS1144

Plasmid

#29060

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple4 (ADORA2A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1142

Plasmid

#29058

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple2 (ADORA2A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

Plasmid

#209081

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

A53T, NAC deletion (aa60-95)

Promoter

TRE4G

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_EML4:1-981

Plasmid

#211315

Purpose

Mammalian expression of full-length human EML4. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EML4:M1-S981 (EML4 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

I382V natural variant (VAR_031727)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP

Plasmid

#192287

Purpose

Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4 (GBX2, CDX2, CDX1, CDX4 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_DCAF12:1-453

Plasmid

#210966

Purpose

Mammalian expression of full-length human DCAF12. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF12:M1-S453 (DCAF12 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS1473

Plasmid

#29234

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple3 (ADORA2A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1143

Plasmid

#29059

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple3 (ADORA2A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_EML4:1-981

Plasmid

#211241

Purpose

Mammalian expression of full-length human EML4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EML4:M1-S981 (EML4 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

I382V natural variant (VAR_031727)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF12:1-453

Plasmid

#211198

Purpose

Mammalian expression of full-length human DCAF12 N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF12:M1-S453 (DCAF12 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_DCAF12:1-453

Plasmid

#211272

Purpose

Mammalian expression of full-length human DCAF12. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF12:M1-S453 (DCAF12 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_DCAF12:1-453

Plasmid

#210967

Purpose

Mammalian expression of full-length human DCAF12. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF12:M1-S453 (DCAF12 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-GBX2-CDX124-Ef1a-mCherry-Neo

Plasmid

#192823

Purpose

Expresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

mCherry and sgRNA CDX1 CDX2 CDX4 and GBX2 (CDX2, CDX1, CDX4, GBX2 Synthetic)

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFBOH-avi-TEV-LIC_DCAF12:1-453

Plasmid

#210940

Purpose

Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF12:M1-S453 (DCAF12 Human)

Use

Tags

Expression

Insect

Mutation

R131Q natural variant (VAR_035322)

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EML4:250-877

Plasmid

#210914

Purpose

Insect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EML4:I250-A877 (EML4 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

K283E natural variant (VAR_031726), I382V Natural…

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG2abc_hsHAUScomplex_FL

Plasmid

#202203

Purpose

Baculoviral transfer vector to co-express 8 subunits of human augmin complex

Depositor

Thomas Surrey

Article

Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109

Inserts

HAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)

Use

Tags

-mGFP-TEV-TwinStrep and His-tag (6x)

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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CHyMErA Exon-deletion hgRNA Library

Pooled Library

#155200

Purpose

Hybrid guide RNA library targeting human alternative cassette exons for deletion and disruption

Depositor

Jason Moffat, Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only
CHyMErA Paralog&Dual-targeting hgRNA Library

Pooled Library

#155199

Purpose

Hybrid guide RNA library targetings human paralog pairs and other protein-coding genes.

Depositor

Jason Moffat, Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Validation (mVAL) CRISPR Library

Pooled Library

#159391

Purpose

Pooled CRISPR library targets core intrinsic immune evasion genes

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Toronto KnockOut (mTKO) CRISPR Library - lentiCRISPRv2

Pooled Library

#159392

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only