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Plasmid#175379PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDN626
Plasmid#175380PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Sgd1
Plasmid#100000PurposeShuttle Sgd1 into Gateway destination vectorDepositorInsertSgd1 (SGD1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Bms1
Plasmid#100001PurposeShuttle Bms1 into Gateway destination vectorDepositorInsertBms1 (BMS1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Ckb1
Plasmid#99981PurposeShuttle yeast Ckb1 into Gateway destination vectorDepositorInsertCkb1 (CKB1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Ckb2
Plasmid#99982PurposeShuttle yeast Ckb2 into Gateway destination vectorDepositorInsertCkb2 (CKB2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Utp11
Plasmid#99984PurposeShuttle yeast Utp11 into Gateway destination vectorDepositorInsertUtp11 (UTP11 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Pfa1
Plasmid#99999PurposeShuttle yeast Pfa1 into Gateway destination vectorDepositorInsertPfa1 (SQS1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Cka1
Plasmid#99979PurposeShuttle yeast Cka1 into Gateway destination vectorDepositorInsertCka1 (CKA1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNJB99
Plasmid#51521PurposeGateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequenceDepositorInsertZif268:FokI
UseGateway entry vectorTagsNLSExpressionMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYHY2
Plasmid#216108PurposeConstitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT0294 signal peptideExpressionBacterialMutationPromoterBfP1E6 promoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRH030
Plasmid#159633PurposeCRISPR‐Cas9 tool for the genetic engineering of Bacillus phagesDepositorTypeEmpty backboneUseCRISPR; Shuttle vectorTagsExpressionMutationPromoterConstitutive tracrRNAAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMO719
Plasmid#117479PurposeDesulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRP2365
Plasmid#127575PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2370
Plasmid#127576PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD57
Plasmid#119882PurposeConstitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpConstitutive_GFP+
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2371
Plasmid#127577PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJYDC1
Plasmid#162458PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the NterminusDepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHA tag, HDEL sequence, eUnaG2 fluorescent protein…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only