Addgene

We narrowed to 5,233 results for: pCas

Showing: 2061 - 2080 of 5233 results

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pCAGGS-SMS2-TEV-Twin-Strep

Plasmid

#202529

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Insert

SGMS2 (SGMS2 Human)

Use

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted stop codon (TGA)

Promoter

CAG and chicken β-actin promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-roxSTOProx-tRFP-pA-lox

Plasmid

#196888

Purpose

Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

turbo (t)RFP

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCA11-pMa-sgTS3-17xMS2V5-sgTS3

Plasmid

#199442

Purpose

CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

HITI donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG CAG CCL20-pre-mGRASP

Plasmid

#173114

Purpose

Expresses CCL20 sender construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCL20-pre-mGRASP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

Plasmid

#182126

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Plasmid

#182121

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP

Plasmid

#187647

Purpose

Encodes gRNA to correct the SFTPC I73T mutation

Depositor

Darrell Kotton

Article

Alysandratos et al Cell Rep. 2021 Aug 31;36(9):109636. doi:

Insert

I73T gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-D830A/E831A

Plasmid

#188085

Purpose

Expresses human ATG9-D830A/E831A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

D830A/E831A

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (692-839)

Plasmid

#188092

Purpose

To make monomeric ATG9 C-terminal tail for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (801-839)

Plasmid

#188093

Purpose

To make monomeric ATG9 C-terminal tail for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-K18D

Plasmid

#188096

Purpose

Expresses human ATG13 HORMA K18D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-K18D

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-K15D

Plasmid

#188094

Purpose

Expresses human ATG13 HORMA K15D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-W50D

Plasmid

#188098

Purpose

Expresses human ATG13 HORMA W50D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-W50D

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-Y152D

Plasmid

#188103

Purpose

Expresses human ATG13 HORMA Y152D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-Y152D

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-R153D

Plasmid

#188104

Purpose

Expresses human ATG13 HORMA R153D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-R153D

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-F16D

Plasmid

#188095

Purpose

Expresses human ATG13 HORMA F16D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-F16D

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-Y118D

Plasmid

#188101

Purpose

Expresses human ATG13 HORMA Y118D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-Y118D

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-E83L

Plasmid

#188099

Purpose

Expresses human ATG13 HORMA E83L mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-E83L

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-Y115D

Plasmid

#188100

Purpose

Expresses human ATG13 HORMA Y115D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-Y115D

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)-F19D

Plasmid

#188097

Purpose

Expresses human ATG13 HORMA F19D mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)-F19D

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Showing: 2061 - 2080 of 5233 results

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