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Plasmid#110582PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertLac promoter with lac operator and RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BpiI restriction sites were r…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D2.0
Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL11057
Plasmid#68253PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barleyDepositorInsertPromote:TaU6+sgRNA HvPM19_1
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTK205
Plasmid#110587PurposeBTK Type 3 coding sequence for golden gate assemblyDepositorInsertGFP optim-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK305
Plasmid#110594PurposeBTK Type 4 terminator for golden gate assemblyDepositorInsertT7 terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLaNGo11-3
Plasmid#126054PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-1
Plasmid#126056PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG113
Plasmid#133124PurposepL0M-PU-NoP, promotorless region used as negative controlDepositorInsertNoP promotorless region from plasmid pIJJ11268
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL80037
Plasmid#68260PurposeLevel 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)DepositorInsertCoding sequence, neomycin phophotransferase II (Escherichia coli)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL11058
Plasmid#68254PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barleyDepositorInsertPromoter_TaU6 + sgRNA_HvPM19_3
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-24xMS2
Plasmid#212629PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorArticleInsertEF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-2xMS2
Plasmid#212627PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorArticleInsertEF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-12xMS2
Plasmid#212628PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorArticleInsertEF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-4xMS2
Plasmid#212626PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorArticleInsertEF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3346 PB-nicking guide-blast
Plasmid#226665PurposePiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidinDepositorInsertprotective nicking sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
miRFP670-TRF1
Plasmid#227388PurposeLabel telomere, no adhesionDepositorInsertTelomeric miRFP (TERF1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only