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Plasmid#186960PurposeER lumen localizaing HaloTagDepositorInsertCALR (CALR Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL2669 (pTnsABf_Vch)
Plasmid#200897PurposeExpresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.DepositorInsertVchTnsA-TnsB
UseTagsInternal NLS between TnsA-TnsB fusionExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLOX-CW-CRE
Plasmid#12238DepositorInsertCRE
UseCre/Lox and LentiviralTagsExpressionMammalianMutationCMV promoter is upstream of nls-CRE. LoxP in 3…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
REEP5-mEmerald
Plasmid#186964PurposeExpress REEP5/DP1 with C-terminus mEmeraldDepositorInsertReep5 (Reep5 Mouse)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1pr_v2-RT
Plasmid#97088PurposeEncodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.DepositorInsertNEAT1pr_v2-RT (NEAT1 Human)
UseCRISPRTagsEYFPExpressionMutationPromoterCMVAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGS36 (GFP-ARF16-PB1)
Plasmid#126581PurposeConstruct to express GFP-ARF16-PB1 under the control of the CMV promoterDepositorInsertOsARF16-PB1 domain
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UGControl
Plasmid#133875PurposeExpresses UGControl in mammalian cellsDepositorInsertUtrCH (UTRN Human)
UseTagsflexible linker - mEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)
Plasmid#53959Purposeallows Cre-mediated expression of transgeneDepositorTypeEmpty backboneUseCre/LoxTagsLox-CAT-LoxExpressionMammalianMutationPromoterCMV enhancer, chicken actin promoterAvailable sinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
POLArIS_NbVim-cp10GFP
Plasmid#171020PurposeExpresses NbVim-cp10GFP#2 in mammalian cellsDepositorInsertanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
UseTagscp10GFPExpressionBacterial and MammalianMutationPromoterCMV promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc hSeparase
Plasmid#59820PurposeAllows the integration of myc hSeparase in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT-mCherry2-G
Plasmid#164169PurposeExpresses mCherry2 under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backboneDepositorInsertmCherry2
UseAAVTagsExpressionMammalianMutationPromotertetOAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase C2029A
Plasmid#59822PurposeAllows the integration of myc Separase C2029A in the genome and Tet-inducible expressionDepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationC2029APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-G
Plasmid#40027DepositorInsertsGFP exon 1
GFP exon 2
beta-globin intron
UseTagsExpressionMammalianMutationinsertion of a loxP site, nucleotides 1-274, and …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Hygro/pTER shLUC (w607-1)
Plasmid#20958Purpose3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, HygroDepositorInsertFirefly luciferase shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP MPS1 K-R
Plasmid#59819PurposeAllows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.DepositorInsertMps1 (TTK Human)
UseTagsEmGFPExpressionMammalianMutationKD, catalytically inactive, D664APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
a5-ecto-pcDNA31hygro
Plasmid#113386PurposeExpress human alpha5 ectodomain with secretion peptide, purification tags and C-terminal claspDepositorInserthuman alpha 5 ectodomain
UseTagsStrep II tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT-mWasabi-G
Plasmid#164170PurposeExpresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backboneDepositorInsertmWasabi
UseAAVTagsExpressionMammalianMutationPromotertetOAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_E1770Q-talin1 (1-2541)
Plasmid#166121PurposeFor expression of full-length mouse talin-1 carrying mutation E1770Q in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertE1770Q-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationE1770QPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron-G
Plasmid#36887DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
sgFFL
Plasmid#59877Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.DepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only