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Plasmid#176139PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassetteDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only
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pSLIK-NFLAG-hEWSR1WT-CMyc
Plasmid#50733PurposeProduces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
DDR_MKOv4
Pooled Library#140219PurposeCRISPR DNA damage response libraryDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSLIK-NFLAG-hEWSR1G511A-CMyc
Plasmid#50734PurposeProduces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 G511A (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Gly 511 to AlaPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1P552L-CMyc
Plasmid#50736PurposeProduces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 P552L (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Pro 552 to LeuPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)
Plasmid#176141PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassetteDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationMyc-tagged PolB with mutation in Asp256 to Ala, a…PromoterEF1AAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211344PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211342PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211343PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc wt MBS1-4
Plasmid#16564DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc mut MBS1
Plasmid#16566DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant MBS1PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc mut MBS2
Plasmid#16567DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant MBS2PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc mut MBS4
Plasmid#16570DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant MBS4PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
DHIP1
Bacterial Strain#226851PurposeExpresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteriaDepositorBacterial ResistanceStreptomycinSpeciesEscherichia coliAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
HL 6845
Bacterial Strain#69785PurposeMC4100 + agn43 coding sequence replaced by T7RNAP and camR + Δdam + ΔSgrSDepositorBacterial ResistanceChloramphenicolAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
HL 6846
Bacterial Strain#69786PurposeMC4100 + agn43 coding sequence replaced by T7RNAP and camR + ΔOxyR + ΔSgrSDepositorBacterial ResistanceChloramphenicolAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)
Plasmid#176140PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resisDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationMyc-tagged PolB with mutations Lys35 to Ala, Lys6…PromoterEF1AAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc mut MBS1-4
Plasmid#16565DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant MBS1-4PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only