Addgene

We narrowed to 4,570 results for: Drosophila

Showing: 2061 - 2080 of 4570 results

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pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124434

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 Exons 1-3

Plasmid

#91799

Purpose

Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

Laccase2 (stw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Availability

Academic Institutions and Nonprofits only

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CS2-CHY-dLIS1

Plasmid

#47045

Purpose

Expression of fluorescent Drosophila LIS1

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Use

Tags

CHY

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
CS2-Myc-dLIS1

Plasmid

#47047

Purpose

Expression of tagged Drosophila LIS1

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pMCh-695

Plasmid

#92263

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 PAM2 mut (gw Fly)

Use

Tags

NHA

Expression

Insect

Mutation

The following point mutations were introduced: EF…

Promoter

Ac5

Availability

Academic Institutions and Nonprofits only

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pMCh-623

Plasmid

#92266

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 6W6A (gw Fly)

Use

Tags

NHA

Expression

Insect

Mutation

The following point mutations were introduced W23…

Promoter

Ac5

Availability

Academic Institutions and Nonprofits only

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pMCh-646

Plasmid

#92289

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 CED 7W7A (gw Fly)

Use

Tags

NHA

Expression

Insect

Mutation

dGW182 CED: aa 940-1215 with the following point …

Promoter

Ac5

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn1-3 (CG11739)

Plasmid

#110642

Purpose

Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG11739 (CG11739 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Promoter

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Use

Tags

6xHis and V5

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS11 AA 300-597

Plasmid

#199329

Purpose

Expresses His-tagged Drosophila IntS11 amino acids 300-597

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 11 (IntS11 Fly)

Use

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 300-597 only

Promoter

Availability

Academic Institutions and Nonprofits only

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pMtnA FLAG-IntS7 puro

Plasmid

#208405

Purpose

Expresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

pMT FLAG MCS puro (defl Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Availability

Academic Institutions and Nonprofits only

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pMtnA FLAG-IntS13 puro

Plasmid

#208406

Purpose

Expresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS13 (asun Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Availability

Academic Institutions and Nonprofits only

Enlarge
pMtnA FLAG-IntS14 puro

Plasmid

#208407

Purpose

Expresses FLAG-tagged Drosophila IntS14 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS14 (IntS14 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Availability

Academic Institutions and Nonprofits only

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SGyA

Plasmid

#160292

Purpose

Drives expression of Cas9 and a TdTomato fluorescent marker

Depositor

Omar Akbari

Article

Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

Inserts

Vas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome

Use

Tags

T2A GFP

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS6 AA 1035-1284

Plasmid

#196904

Purpose

Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 6 (IntS6 Fly)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS8 AA 1-308

Plasmid

#196905

Purpose

Expresses His-tagged Drosophila IntS8 amino acids 1-308

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 8 (IntS8 Fly)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-IntS6 AA 1-400

Plasmid

#195071

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1-400

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-IntS6 AA 1-600

Plasmid

#195072

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1-600

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-IntS6 AA 101-1284

Plasmid

#195073

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 101-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Availability

Academic Institutions and Nonprofits only

Enlarge
pUb FLAG-IntS6 AA 1197-1284

Plasmid

#195074

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1197-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ubi-p63e

Availability

Academic Institutions and Nonprofits only

Enlarge