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Plasmid#59356PurposeKnocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsGFPExpressionMammalianMutationSilent mutations in amino acids 30-33 to confer s…PromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH1R-P2A-mCherry-N1
Plasmid#84330PurposeCo-expresses (untagged) Histamine-1 receptor and mCherryDepositorInsertH1R-P2A-mCherry (HRH1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pblic-neo TRX1
Plasmid#110919PurposeTo overexpress Thioredoxin-1DepositorInsertTRX1 (TXN Human)
UseRetroviralTagsExpressionMutationPromoterunknownAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mBATF
Plasmid#34575DepositorInsertBATF (Batf Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-E2F2
Plasmid#202521PurposeExpressing human E2F2 protein with FLAG-tagDepositorInsertE2F2 (E2F2 Human)
UseTagsFLAG-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-E2F1
Plasmid#202520PurposeExpressing human E2F1 protein with FLAG-tagDepositorInsertE2F1 (E2F1 Human)
UseTagsFLAG-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-tat:msfGFP
Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVS102_P3-GFP-NATMX6
Plasmid#99048PurposeEGFP reporter with six binding sites for the Zif268 DNA binding domainDepositorInsertsMcIsaac 2014 P3 promoter
eGFP
clonNAT resistance
UseTagsExpressionYeastMutationGAL1 promoter with 4 GAL4 sites removed and repla…PromoterMcIsaac 2014 P3 promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH4R-mCherry-N1
Plasmid#84328PurposeHistamine-4 receptor tagged with mCherryDepositorInsertH4R-mCherry (HRH4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIRCgp41-1
Plasmid#74227PurposeVector for SICLOPPS-mediated protein circularization using the gp41-1 intein.DepositorTypeEmpty backboneUseSynthetic BiologyTagsgp41-1 C-intein and gp41-1 N-inteinExpressionBacterialMutationPromoterT7-lacAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 (-756/+68) SBR2 Mut 3' box
Plasmid#15711DepositorInsertp15 promoter (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMut 3' box in SBR2. The 3' box contains…PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
FpG5.BFP.Reporter
Plasmid#134967PurposeLentiviral vector for enhancer reporter assayDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromotermini Fgf4 promoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag
Plasmid#154970PurposeExpresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.DepositorInsertPfEBA175
UseTagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationinsert codes for EBA175 residues 1284 to 1462PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-ASF1B
Plasmid#31205DepositorInsertASF1B (ASF1B Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartatePromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH2R-P2A-mCherry-N1
Plasmid#84331PurposeCo-expresses (untagged) Histamine-2 receptor and mCherryDepositorInsertH2R-P2A-mCherry (HRH2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT5T_hp53_ST
Plasmid#34922DepositorInsertp53 DNA-binding (Res 1-393) (TP53 Human)
UseTagsExpressionBacterialMutationC135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …PromoterT7Available sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆PABC
Plasmid#81064PurposeGateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
UseCloningTagsExpressionMutationY2402A and K2415A (bp t7204g, a7205c, t7206c, a72…PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only