-
Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6sgp53-U6sgbbSapI-GFAPCre
Plasmid#100275PurposeAAV-CRISPR mediated genome editing in glia / astrocytesDepositorTypeEmpty backboneUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…TagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i2 ONE-GO
Plasmid#189736PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTDH-His-bio
Plasmid#52331PurposeExpresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertMTDH (MTDH Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-ABE8e-P2A-GFP
Plasmid#178177PurposeIVT template plasmid for making ABE8e-P2A-GFP modRNADepositorInsertABE8e
UseIvt template plasmidTagsP2A-GFPExpressionMutationPromoterT7 promoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP617_αGCN4-mCherry
Plasmid#211785PurposeSunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selectionDepositorInsertSnoopCatcher-DNMT3A
UseTags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP777_SnoopCatcher-p65HSF1-CO
Plasmid#211770PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertSnoopCatcher-p65HSF1
UseTags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
B2342_BTB-mCherry
Plasmid#215095PurposeExpresses BCL6 BTB(1-129)-mCherry in mammalian cellsDepositorInsertBCL6 BTB (BCL6 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-SpCas9-BlastR
Plasmid#107190PurposeConfers constitutive expression of SpCas9DepositorInsertSpCas9 (NEWENTRY )
UseCRISPRTags3xFLAGExpressionBacterial and MammalianMutationPromoterChicken ß-actinAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP618_αGCN4-KRAB-CO
Plasmid#211782PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertaGCN4-mCherry
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect mCherr…PromoterpEF1a and pSV40Available sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PKCdelta-EGFP
Plasmid#178856PurposeMammalian expression of PKCδ fused to EGFPDepositorInsertPKCδ (PRKCD Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-7
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialMutationPromoterP(rhaB)Available sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
VTN-bio-His
Plasmid#53429PurposeExpresses full-length Vitronectin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertVTN (VTN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
B2396_BTB-EGFP
Plasmid#215099PurposeExpresses BCL6 BTB(1-129)-EGFP in mammalian cellsDepositorInsertBTB-EGFP (BCL6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]
Plasmid#227245PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP788_Traptavidin-EZH2-FL-CO
Plasmid#211781PurposeAviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-EZH2
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
SELP-bio-His
Plasmid#51639PurposeExpresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSELP (SELP Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only