Addgene

We narrowed to 13,574 results for: pET

Showing: 2041 - 2060 of 13574 results

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pET29NK-LGK/mGFPmut3

Plasmid

#81168

Purpose

GFP fusion to levoglucosan kinase

Depositor

Timothy Whitehead

Article

Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

Insert

Levoglucosan kinase wild-type

Use

Tags

His6x and mGFPmut3

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-239

Plasmid

#74609

Purpose

Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239 (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pET22b-ecDHFR-G51PEKN

Plasmid

#118582

Purpose

Bacterial expression of E.coli DHFR G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

G51 mutated to P51; Glu-Lys-Asn residues inserted…

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-p52(1-398)

Plasmid

#44733

Depositor

Gourisankar Ghosh

Insert

p52 (NFKB2 Human)

Use

Tags

Expression

Bacterial

Mutation

AA 1-398

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pET51b(+)_MBP-nLucCP-67/68

Plasmid

#113922

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-67/68

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 G399S

Plasmid

#16158

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

G399S

Promoter

T7

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET28b-sad1-C63A

Plasmid

#90302

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Use

Tags

His tag

Expression

Bacterial

Mutation

C63A

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-GST

Plasmid

#51528

Purpose

Express GST-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p40)

Plasmid

#135940

Purpose

Expresses AUF1p40 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b Me31B-3Myc

Plasmid

#113001

Purpose

For protein expression and purification of Myc-tagged full-length Drosophila me31B

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length me31B (me31B Fly)

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MDC

Plasmid

#108950

Purpose

heterologous expression of MDC of S. cerevisiae in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate decarboxylase (MDC)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-PMK

Plasmid

#108949

Purpose

heterologous expression of PMK of E. faecalis in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Phosphomevalonate kinase (PMK)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-IDI2

Plasmid

#108951

Purpose

heterologous expression of IDI2 of T. thermophilus in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Isopentenyldiphosphat isomerase 2 (IDI2)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET28 mSmo-Cterm

Plasmid

#58703

Purpose

used for anti-mouse Smoothened antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

6X-HIS

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
pET29b-Ava3858-3857

Plasmid

#45744

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3857

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 S400D

Plasmid

#16156

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S400D

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET-28a(+)-AACT

Plasmid

#108944

Purpose

heterologous expression of AACT of M. sedula in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Acetoacetyl-CoA synthase (AACT)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-T-protein

Plasmid

#12652

Depositor

SeWon Suh

Article

Lee et al J Biol Chem. 2004 Nov 26. 279(48):50514-

Insert

T-protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-deltaPYD

Plasmid

#51522

Purpose

Express His6-tagged HIN domain of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

deletion of pyrin domain

Promoter

lac operator

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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