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We narrowed to 13,819 results for: pET

Showing: 2041 - 2060 of 13819 results

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pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-W318R

Plasmid

#174793

Purpose

Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-W318R (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

W318, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a- hPTP1e-PDZ2

Plasmid

#20377

Depositor

Andrew Lee

Article

Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

Insert

hPTP1e PDZ2 (PTPN13 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RFP-RGG

Plasmid

#124944

Purpose

Exoression of RGG-RFP-RGG

Depositor

Matthew Good, Benjamin Schuster, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His and mCherry

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-IS200

Plasmid

#12604

Depositor

SeWon Suh

Article

Lee et al J Biol Chem. 2006 Feb 17. 281(7):4261-6.

Insert

IS200 transposase (SSO1474 Sulfolobus solfataricus)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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TrmD_HaeIn/pET28b(+) N,C-tag

Plasmid

#12665

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 200

Insert

tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pETM-30-GST-Dmp68_B

Plasmid

#145962

Purpose

Bacterial Expression of Dmp68

Depositor

Elisa Izaurralde

Insert

Dmp68 (Rm62 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET-HJURP-CBP

Plasmid

#30393

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

HJURP CenpA Binding Domain (CENPA Budding Yeast, Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pET151-mUCH37 (ISF2)

Plasmid

#61928

Purpose

Expression of mUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

UCH37 (M.musculus) (Uchl5 Mouse)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET-15b-PTS

Plasmid

#108953

Purpose

heterologous expression of PTS of P. cablin in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Patchoulol synthase (PTS)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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HOXD9 (human) pET

Plasmid

#8570

Depositor

Corey Largman

Insert

HOXD9 (HOXD9 Human)

Use

Tags

T7

Expression

Bacterial

Mutation

PCR human cDNA clone; makes protein by in vitro T…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pBC2T-Ypet-FtnA

Plasmid

#108969

Purpose

expresses BC2-tag-Ypet-FtnA in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

FtnA (ftnA Escherichia coli)

Use

Tags

BC2-tag and Ypet

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBP-pET-RBS

Plasmid

#72981

Purpose

PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

pET RBS derived from pET vectors

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Article

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Use

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2_rpoA-U

Plasmid

#190956

Purpose

Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3_rpoA-U

Plasmid

#190960

Purpose

Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET12a-KIo18/08 C214S

Plasmid

#47099

Depositor

Marina Ramirez-Alvarado

Insert

KIo18/08 C214S (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET21b - S. thermophilus DltB

Plasmid

#132607

Purpose

expresses S. thermophilus DltB in E. coli

Depositor

Wenqing Xu

Article

Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

Insert

Dltb

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160S)

Plasmid

#66872

Purpose

Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)

Use

Tags

Histag from pET30Xa-LIC

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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