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pAV-U6+27-Tornado-biRhoBAST
Plasmid#196344PurposeOverexpression of circular biRhoBASTDepositorInsertTornado-biRhoBAST
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pREDawn-StrR-MCS
Plasmid#188979PurposeFor red light-induced bacterial expression of gene of interest (StrR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2SK
Plasmid#59383PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.DepositorInsertTT-ISceI-kan
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_ALFA-tag-SNAP
Plasmid#136628PurposePlasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusionDepositorInsertEGFP-NbALFA
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET42a-lambdaN+-L+-GSH
Plasmid#98894PurposeExpresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo methodDepositorInsertlambdaN+-L+
UseTagsGST tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-2xbiRhoBAST
Plasmid#196349PurposeExpression of mEGFP mRNA with 2xbiRhoBAST in 3'UTRDepositorInsertmEGFP-2xbiRhoBAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-YY1
Plasmid#169018PurposeExpression of YY1 driven by CMV promoterDepositorInserthuman YY1 (YY1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZE33 Prickle-1-GFPnopren-pCS2+
Plasmid#17074DepositorInsertPrickle-1 (prickle1a Zebrafish)
UseZebrafish expressionTagsGFPExpressionMutationPk1GFPnopren deletes the C-terminal six amino aci…PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk3
Plasmid#81012PurposeExpression of Zdk1 anchored to mitochondriaDepositorInsertZdk3
UseTags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoNanobody
Plasmid#136622PurposePlasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusionDepositorInsertNTOM20-SNAP-Tag-EnhancerNb
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-L1221
Plasmid#101335Purposenegative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGTDepositorInsertshRNA targeting miR30
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VCre
Plasmid#89575PurposeExpresses VCre under pCAGDepositorInsertVCre recombinase
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_CalR_HaloTag9_KDEL
Plasmid#175540PurposeExpression of HaloTag9 in the endoplasmid reticulum of mammalian cellsDepositorInsertCalR-HaloTag9-KDEL
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
CSII-prEF1a-E2-Crimson-NLS
Plasmid#125263PurposeFluorescent reporter for cell sizeDepositorInsertE2-Crimson-NLS
UseLentiviralTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shNFS1_1
Plasmid#102963PurposeSuppression of NFS1DepositorInsertshNFS1_1 (NFS1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_HaloTag9_LamB1
Plasmid#175538PurposeExpression of HaloTag9 at the nuclear lamina of the mammalian cellsDepositorInsertHaloTag9-LamB1
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
RSRF-Luc-2wt
Plasmid#31818DepositorInsertMEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseTagsExpressionMammalianMutationPromoterMEF2-dependent (-307 to -242 of Nur77/NR4A1)Available sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f-AAV
Plasmid#182501PurposeGFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotorDepositorInsertER localized P450
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only
