Addgene

We narrowed to 5,233 results for: pCas

Showing: 2021 - 2040 of 5233 results

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pCA528 IST1 L328D, L355A (1-366)

Plasmid

#193040

Purpose

Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Use

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L328D, L355A

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 V18D,F98D-OSF

Plasmid

#193045

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Use

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

V18D, F98D

Promoter

Chicken Beta-actin

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 V18D,F98D (1-165)

Plasmid

#193034

Purpose

Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Use

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

V18D, F98D, Residues 1-165

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

Plasmid

#221551

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

gRNA targeting human Rab7A (RAB7A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1

Plasmid

#222141

Purpose

Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

SP6

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

gRNA targeting human Rab7A (RAB7A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCaspase-R153A

Plasmid

#214036

Purpose

Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

R153A

Promoter

T7 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

Plasmid

#213728

Purpose

Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bIV spectrin (repeats 13-15) (Sptbn4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

Plasmid

#213729

Purpose

Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bII spectrin (repeats 13-15) (Spnb3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-mCherry-NAP1 (WT)

Plasmid

#198036

Purpose

Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Use

Tags

GST-TEV and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCaUra3-CUbo(K48R/K63R)-yeGFP

Plasmid

#212774

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (363-450)

Plasmid

#210849

Purpose

Mammalian expression of ATG13 (363-450) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-R334D-TSF

Plasmid

#210841

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

R334D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-R326D-TSF

Plasmid

#210840

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

R326D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-V314D-TSF

Plasmid

#210839

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

V314D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-V44D-TSF

Plasmid

#210843

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

V44D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaR-SpCas9-NLS-6xHis

Plasmid

#207388

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A, amino acids 10…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-NLS-6xHis

Plasmid

#207382

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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