Addgene

We narrowed to 2,325 results for: gem

Showing: 2021 - 2040 of 2325 results

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pSLI-sandwich(0807600)

Plasmid

#85790

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R

Use

P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pFP.R269

Plasmid

#138181

Purpose

Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R272

Plasmid

#138221

Purpose

Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Barx1

Plasmid

#84306

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Barx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Barx1 (Barx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFP.R270

Plasmid

#138219

Purpose

Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R275

Plasmid

#138224

Purpose

Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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PROM5_MpEF1a

Plasmid

#136113

Purpose

Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpEF1a Mapoly0024s0116

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pISH-En2

Plasmid

#84310

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

En2 (En2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFP.R371

Plasmid

#138241

Purpose

Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R400

Plasmid

#138175

Purpose

Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pTC223

Plasmid

#70019

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSLI-N-sandwich-loxP(K13)

Plasmid

#85793

Purpose

P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

double 2xFKBP, GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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His10-tag

Plasmid

#55179

Purpose

10xHis-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

His10

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBW103ParsR-Amp30C

Plasmid

#78638

Purpose

pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-30hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDsRed (GB0100)

Plasmid

#68202

Purpose

Provides the CDS of DsRed as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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cMyc-tag

Plasmid

#55183

Purpose

cMyc tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

cMyc tag

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYFP (GB0053)

Plasmid

#68197

Purpose

Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

YFP coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCngA2-ISH

Plasmid

#15855

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Cyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pFP.R375

Plasmid

#138245

Purpose

Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge