Addgene

We narrowed to 27,942 results for: eng

Showing: 2021 - 2040 of 27942 results

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pCAG-Positron

Plasmid

#129253

Purpose

Express Positron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron

Use

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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MCP-M-MLV-RT

Plasmid

#181799

Purpose

MCP-M-MLV-RT (MS2-N55K-MMLVRT)

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MCP-M-MLV-RT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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U6-petRNA

Plasmid

#181802

Purpose

petRNA cloning plasmid

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

petRNA-Kan

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSET-dCas9

Plasmid

#110183

Purpose

Expression of the dCas9 gene in Streptomyces

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Insert

dCas9

Use

CRISPR; I

Tags

Expression

Bacterial

Mutation

D10A, H840A

Promoter

ermE*p

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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EndophilinA1-mRuby2

Plasmid

#171945

Purpose

Expresses Endophilin A1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Endophilin A1 (SH3GL2 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1-v5.1+T1-empty

Plasmid

#204977

Purpose

AsCas12f1-v5.1+T1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nature Catalysis (2023) 6, 695–709

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

N70Q/K103R/A104R/S118A/D364R

Promoter

PCMV,U6

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3

Plasmid

#204808

Purpose

Bacterial co-expression of Dengue2 NS2B and NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue2 NS2B (POLY Dengue2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-NLS-M-MLV-RT

Plasmid

#181804

Purpose

pET28a-NLS-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

NLS-M-MLV-RT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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PE-GsI-IIC-RT

Plasmid

#181808

Purpose

PE-GsI-IIC RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

PE-GsI-IIC-RT

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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scFv-M-MLV-RT

Plasmid

#181809

Purpose

scFv-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MMLV_RT

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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Skylan-S-N1

Plasmid

#129737

Purpose

A green photo-switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

Depositor

Pingyong Xu

Article

Zhang et al ACS Nano. 2015 Mar 24;9(3):2659-67. doi:

Type

Empty backbone

Use

Tags

Skylan-S

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJT11F

Plasmid

#165062

Purpose

Introducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

T-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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petRNA-FANCF-v3'

Plasmid

#181803

Purpose

petRNA FANCF v3'

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

ribozyme-petRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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MS2-pegRNA

Plasmid

#181811

Purpose

ms2 incorporated pegRNA-1.1

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MS2-pegRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72103

Plasmid

#40780

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi + cin + repL + pacA

Use

Synthetic Biology; Phagemid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-human Beta-catenin R1C (138–781)

Plasmid

#132608

Purpose

expresses human beta-catenin R1C (138–781) in E. coli

Depositor

Wenqing Xu

Article

Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

Insert

Beta-catenin R1C (138–781)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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C-12-BE

Plasmid

#135040

Purpose

C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L2_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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N-8-BE

Plasmid

#135023

Purpose

C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJAID8Z

Plasmid

#165053

Purpose

Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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