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Plasmid#207113PurposeHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only
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mEGFP-Rac1
Plasmid#83950PurposeRac1 FLIM donor for dynamic imaging of GTPase activityDepositorInsertras-related C3 botulinum toxin substrate 1 (RAC1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-mCherry
Plasmid#80946Purposehuman AAVS1 site targeting donor plasmid for knocking-in mCherry expression cassette driven by CAG promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHD-DsRed
Plasmid#51434PurposeVector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.DepositorInsertLoxP-3xP3-DsRed-LoxP
UseTagsExpressionMutationPromoter3xP3Available sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTLR repair vector
Plasmid#64322PurposeVector for repair of the traffic light reporter (TLR), providing the venus coding regionDepositorInsertVenus
UseArtificial targeting vectorTagsExpressionMutationsee publication for detailsPromoternoneAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH2B
Plasmid#133390PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-64: FUS-mEGFP
Plasmid#114410PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human FUSDepositorInsertFUS Homology Arms with linker-mEGFP (FUS Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 5, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAVS1-Puro Xlone-eGFP
Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorInsertall-in-one tet-on system
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nanog-2A-mCherry
Plasmid#48680PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorInsertNanog (Nanog Mouse)
UseCRISPRTagsCherryExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLS.491
Plasmid#184956PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
UseTagsExpressionBacterialMutationrpoB donor T1534CPromoterT7/lacAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYZ145
Plasmid#98405PurposeDonor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ149
Plasmid#98407PurposeDonor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-55:DMD-mEGFP
Plasmid#159744PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertDMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-eGFP-P2A-dTAG-Ing3
Plasmid#170754PurposeDonor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locusDepositorInserteGFP-P2A-2xHA-dTAG
UseMouse TargetingTagseGFP-P2A-2xHA-dTAGExpressionMutationPhenylalanine 36 to valine in FKBP12PromoternoneAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMN-009
Plasmid#195413PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF1DepositorInsert4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLQ-KO-tgt50
Plasmid#126578PurposeCRISPR-Cas9 based efficient genome editing in S. aureusDepositorInsertCas9-Pxyltet-sgRNA-Pspac-donor-tgt50
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMN-018
Plasmid#195422PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF10DepositorInsert4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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mCyRFP1-RhoA
Plasmid#84358Purposefusion protein of RhoA, FRET donorDepositorInsertmCyRFP1-RhoA (RHOA Synthetic, Human)
UseTagsmCyRFP1 N terminal fusion to RhoAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only