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Plasmid#91166PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt IIDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3F2
Plasmid#73428PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.DepositorInsertRepressor C4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-CjCAS9-pA
Plasmid#131543PurposeExpresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCjCas9
UseCRISPRTagsFLAG-SV40NLS, SV40NLS, and polyAExpressionMammalianMutationHuman codon-optimizedPromoterCAG, T3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_1
Plasmid#91162PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
peSpdCas9-J23109
Plasmid#113153PurposePlasmid constitutively expressing eSpdCas9 proteinDepositorInserteSpdCas9
UseTagsExpressionBacterialMutationD10A & K810A & H840A & K1003A & R…PromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAflaB
Plasmid#149642Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAmreB
Plasmid#149653Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x6
Plasmid#63600PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD1361YPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
phumanCas9 (GB0575)
Plasmid#68206PurposeProvides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid partDepositorInsertCas9 coding region (human codon optimised)
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removed; human codon optimis…PromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-dead_1
Plasmid#91165PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA
Plasmid#136659PurposeExpresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNADepositorInsertsUseCRISPR and Synthetic BiologyTags2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9ExpressionMammalianMutationPromoterCAG and U6Available sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:PtFCP:Cas9YFP
Plasmid#104894PurposeCas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformationDepositorInsertCas9
UseCRISPR; ; golden gate assemblyTagsYFPExpressionMutationPromoterAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_SaCas9_sgRNA2
Plasmid#107330PurposeU6 driven SaCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
KA2706_eSpCas9-PGKPacdtkCh
Plasmid#124204PurposeExpression of eSpCas9(1.1) and sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XL)Cas9
Plasmid#127386PurposeExpresses Cas9 at very low levelDepositorInsertuORF(XL)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_2
Plasmid#91163PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9- AsiA_m1.1
Plasmid#158064PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 1.1DepositorInsertdCas9-AsiA_m1.1
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9-L2
Plasmid#141038PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Topo_SpCas9.tracr_AsCas12a.DR
Plasmid#155050PurposeTOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vectorDepositorInsertSpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMST665_BB3_L 23_gRNAempty_cas9_BbsI
Plasmid#90278PurposeBB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9DepositorInsertCas9
UseA. nigerTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only