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Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…TagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R274
Plasmid#138223PurposeExpresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R401
Plasmid#138222PurposeExpresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R360
Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-mRFP
Plasmid#128126PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen (benzoate responsive promoter)Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-Hyg
Plasmid#210261PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKL003
Plasmid#98919PurposeConstitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.DepositorInsertPROPS
UseTagsHisExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
placZα-tdMCP
Plasmid#191132PurposeExpression of tdMCP from a lacZα inducible promoterDepositorInsertdMCP_1
UseSynthetic BiologyTagsExpressionMutationPromoterlac-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-tag
Plasmid#55182PurposeHA-tagDepositorInsertHA tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKL004
Plasmid#98920PurposeConstitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.DepositorInsertGCaMP6f tethered to mRuby
UseTagsHis and mRuby3ExpressionBacterialMutationPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R267
Plasmid#138179PurposeExpresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
1.3xWT HBV-Luciferase
Plasmid#61549PurposeThe 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of CoreDepositorInsert1.3 times of the HBV genome with luciferase within the Core ORF
UseLuciferaseTagsExpressionMammalianMutationSubstitution of HBV sequences with luciferase ORF…PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTnos (GB0037)
Plasmid#68188PurposeProvides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid partDepositorInsertNos terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLI-2xFKBP-GFP(1463000)
Plasmid#85789PurposeP. falciparum integration plasmid to fuse endogenous genes with tags using SLIDepositorInsertPF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
UseP. falciparum integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-iresFUCCI
Plasmid#133371PurposePiggybac vector for constitutive expressionDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPnos (GB0072)
Plasmid#68166PurposeProvides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid partDepositorInsertNos promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP.R268
Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-tag
Plasmid#55180PurposeFLAG-tagDepositorInsertFLAG
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBFP (GB0025)
Plasmid#68193PurposeProvides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid partDepositorInsertBFP coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only