Addgene

We narrowed to 18,998 results for: tes

Showing: 1981 - 2000 of 18998 results

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pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

Plasmid

#208400

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Baptiste Pradel et al bioRxiv

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2

Plasmid

#133980

Purpose

inducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

deletion of MIU1 and MIU2

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Tet-off TauRD (P301L/V337M)

Plasmid

#188572

Purpose

Dox-repressible expression of the Tau repeat domain

Depositor

Franz-Ulrich Hartl

Article

Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

Insert

Tau repeat domain (MAPT Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

P301L and V337M

Promoter

pTight TRE

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pIRESh-GFPII-MB-Dlk1

Plasmid

#108930

Purpose

GFP-tagged vector expressing membrane-bound Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Use

Tags

GFPII

Expression

Mammalian

Mutation

lacks coding sequence for the juxtamembrane motif…

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRS3 (CK2alpha, CK2beta)

Plasmid

#27092

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

bidirectional tet-responsive promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pIRESh-GFPII-S-Dlk1

Plasmid

#108931

Purpose

GFP-tagged vector expressing secreted isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Use

Tags

GFPII

Expression

Mammalian

Mutation

contains sequences from full length Dlk1A up to t…

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A2-Neo

Plasmid

#189743

Purpose

The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A2 (ALDH1A2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A1-Neo

Plasmid

#189742

Purpose

The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A3-Neo

Plasmid

#189744

Purpose

The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 (ALDH1A3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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cmamxA2-GFP

Plasmid

#107197

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

cmannexin A2 (ANXA2 Human)

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

all type II Ca2+ binding sites deleted ( D161A, E…

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387E

Plasmid

#60653

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mammalian

Mutation

S387E mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387A

Plasmid

#60652

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mutation

S387A mutant

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 5A

Plasmid

#60651

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated five serines to alanines

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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mGFP-hNET-A457P

Plasmid

#193365

Purpose

expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

Depositor

Harald Sitte

Article

Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

Insert

human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)

Use

Tags

monomeric GFP (mGFP)

Expression

Mammalian

Mutation

A457P (GCC to CCC)

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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mGFP-hNET-R121A/K334A/R440A

Plasmid

#193366

Purpose

expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

Depositor

Harald Sitte

Article

Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

Insert

human Norepinephrine Transporter (SLC6A2 Human)

Use

Tags

monomeric GFP (mGFP)

Expression

Mammalian

Mutation

R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 4A

Plasmid

#59907

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated four serines to alanines

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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Tet-off Full length Tau (0N4R, P301L/V337M)

Plasmid

#188573

Purpose

Dox-repressible expression of the 0N4R Tau isoform

Depositor

Franz-Ulrich Hartl

Article

Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

Insert

0N4R Tau (MAPT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P301L and V337M

Promoter

pTight TRE

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGV13 (CK2alpha K68M, CK2beta)

Plasmid

#27094

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha K68M (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA on CK2alpha and Myc on CK2beta

Expression

Mammalian

Mutation

K68M on CK2alpha

Promoter

bidirectional tet-responsive promoter and bidirec…

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRS2 (CK2alpha', CK2beta)

Plasmid

#27093

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha' (CSNK2A2 Human)

Use

Tetracycline-regulated expression

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

bidirectional tet-responsive promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge