Addgene

We narrowed to 12,845 results for: ski

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pRS414 Hsh155 R294L/K335E

Plasmid

#87283

Purpose

Encodes Hsh155 R294L K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

R294L & K335E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 ZFYVE9

Plasmid

#67134

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ZFYVE9 (ZFYVE9 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

F1359I

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Ecm2 1-143

Plasmid

#169923

Purpose

WT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-143

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Ecm2 1-265

Plasmid

#169925

Purpose

WT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-265

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Ecm2 1-325

Plasmid

#169926

Purpose

WT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-325

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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p1071-Lenti-Single loxP

Plasmid

#217892

Purpose

Lentiviral vector, entry vector for Lentiviral Switch-OVER

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLENTI-CMV-GFP-TREX1 D18N BLAST

Plasmid

#164225

Purpose

lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D18N

Promoter

CMV

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GDI2

Plasmid

#67093

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GDI2 (GDI2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
mito-PAGFP

Plasmid

#23348

Purpose

Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

Depositor

Richard Youle

Article

Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

Insert

PAGFP

Use

Tags

mito

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-eBFP2-NLS-pA

Plasmid

#183800

Purpose

AAV vector to express nuclear localized eBFP2 from CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eBFP2-NLS

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTH

Plasmid

#178949

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTH-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37082

Purpose

Cre-dependent expression of hChR2(H134R) for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pET15b/bPanK

Plasmid

#73619

Purpose

Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

Depositor

Suzanne Jackowski, T. J. Kappock

Article

Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

Insert

pantothenate kinase (coaA Escherichia coli)

Use

Tags

His6

Expression

Bacterial

Mutation

relative to MG1655 sequence, changes glutamate-5 …

Promoter

T7

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLX302 OAS2-V5 puro

Plasmid

#158642

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OAS2 (OAS2 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL6

Plasmid

#67061

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL6 (IL6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

U6

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts3

Plasmid

#115864

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts1

Plasmid

#115862

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts2

Plasmid

#115887

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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