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Plasmid#206223PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD
Plasmid#162804PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI_IRES_Blasticidin
UsePiggybacTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mCit-IRES-Neo
Plasmid#155230Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mCitrine sensor
UseLentiviralTagsmCitrineExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-FT-mCitrine-EEVEE-CaaX
Plasmid#155225PurposeMPAct with increased linker flexiblityDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290
Plasmid#129670PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationAacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mc2 neo EF1a
Plasmid#206220PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNV-gfp-IRES-MCS
Plasmid#53122PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; For expression in chicken cellsTagsEGFP-IRESExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZ001 (AacC2c1 locus)
Plasmid#70166PurposeExpresses AacC2c1 full locusDepositorInsertAacC2c1 locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_EcSSPB-nano_X
Plasmid#131212PurposeExpresses EcSSPB-nano (Escherichia coli) followed by restriction sitesDepositorInsertEcSSPB-nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDZ617 pKAN 12xPP7 V4
Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry
Plasmid#206219PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 neo
Plasmid#206214PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC82-P1G10
Plasmid#202923PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82DepositorInsertEGFP/BC82
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_BFP
Plasmid#45297DepositorInsertBFP
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC2-P1A2
Plasmid#202843PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2DepositorInsertEGFP/BC2
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOG603
Plasmid#105356PurposeSynthetic biologyDepositorInsertpromotor RPS5a (At3g11940; 1700bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1-Lyn-mCitrine-EEVEE-LifeAct
Plasmid#155226PurposeAlternate MPAct using Lyn/LifeAct instead of CaaX/F-tractinDepositorInsertLyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC6-P1A6
Plasmid#202847PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6DepositorInsertEGFP/BC6
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAL612
Plasmid#119860Purposeprecusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 CysteineDepositorInsertouter membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
UseTagsnoneExpressionBacterialMutationCysterine 290 to Serine and Glycine 244 to Cystei…PromoterlacAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
VYAIFIFQL_epitope
Plasmid#165018PurposeExpression of CMV US20 (29-37) fused to IL-2 signal sequenceDepositorInsertCMV US20 (29-37)
UseLentiviralTagshuman IL2 signal sequence at N-termExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only