Addgene

We narrowed to 8,318 results for: puc

Showing: 1981 - 2000 of 8318 results

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pAW8ENdeI-6XDSR

Plasmid

#48980

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-4XDSR

Plasmid

#48978

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pNSEN-d2

Plasmid

#59763

Purpose

Expresses nuclear EGFP and Tomato in mammalian cells

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

Inserts

d2-EGFP
Tomato

Use

Tags

3X NLS and PEST

Expression

Mammalian

Mutation

Promoter

CMV promoter and SV40 promoter

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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KG#908

Plasmid

#110914

Purpose

Expresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegans

Depositor

Kenneth Miller

Inserts

rab-3 promoter
TIR1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAA6

Plasmid

#128282

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq- mRFP, parS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE190

Plasmid

#120781

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 1 (G1_mTurquoise)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

G1_mTurquoise

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-spo5DSR

Plasmid

#48983

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP

Plasmid

#48988

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-8XDSR

Plasmid

#48981

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA

Plasmid

#48989

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA tag

Expression

Mutation

Promoter

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-3XDSR

Plasmid

#48976

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pRTVIR

Plasmid

#46365

Depositor

Claes Andréasson

Article

Andreasson et al PLoS One. 2013 Sep 13;8(9):e74207. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pED17x11

Plasmid

#134814

Purpose

sfGFP expression plasmid for characterizing qtRNA charging

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

sfGFP-151-TAGA

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

151-TAGA

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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GGE132

Plasmid

#120745

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-4AB)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_EYK300-4AB

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE104

Plasmid

#120744

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-3AB)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_EYK300-3AB

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa VP64 Afp

Plasmid

#99697

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

Depositor

George Church

Insert

dCas9 and gRNA targeting Afp (Afp Synthetic)

Use

AAV and CRISPR

Tags

VP64

Expression

Mutation

dead Cas9

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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JM109

Bacterial Strain

#49761

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

None

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
JM107

Bacterial Strain

#49759

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
JM106

Bacterial Strain

#49757

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
JM110

Bacterial Strain

#49763

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

Streptomycin

Available since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only