Addgene

We narrowed to 9,991 results for: pcdna3.1

Showing: 1981 - 2000 of 9991 results

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pcDNA3.1-CHRNA7-YFP

Plasmid

#62630

Purpose

mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pY130 (pcDNA3.1-U6-huBsCpf1)

Plasmid

#92306

Purpose

Expression of humanized BsCpf1 and crRNA

Depositor

Feng Zhang

Insert

huBsCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) mMff isoform 5

Plasmid

#44601

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

mMff isoform 5 (Mff Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-695)/pcDNA3.1

Plasmid

#111453

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

8xHis and EGFP

Expression

Mammalian

Mutation

Kv7.4 (1-695)

Promoter

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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CRT-GFP pcDNA3.1

Plasmid

#80978

Purpose

mammalian expression of CRT-GFP

Depositor

Peter Steyger

Article

Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

Insert

CRT (Calr Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

no KDEL

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pY014 (pcDNA3.1-hMbCpf1)

Plasmid

#69986

Purpose

Expresses humanized MbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hMbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
DRD2-3xHA/pCDNA3.1

Plasmid

#196940

Purpose

Plasmid expressing an antigen targeting the D2 dopamine receptor

Depositor

James Trimmer

Insert

DRD2 (DRD2 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-2delta

Plasmid

#124146

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Mfn2-HA S27D

Plasmid

#139194

Purpose

Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

Depositor

Allan Weissman

Article

Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

Insert

MFN2 (MFN2 Human)

Use

Tags

2HA

Expression

Mammalian

Mutation

Serine 27 to Aspartic Acid

Promoter

CMV promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Golgi-RasAR

Plasmid

#205570

Purpose

Golgi membrane-targeted RasAR.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

Golgi-RasAR (RAF1, GOLGB1 Synthetic, Human)

Use

Tags

Golgin B1 (Giantin) residues 3131-3259

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 Sense

Plasmid

#60631

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RaACR_687-EYFP

Plasmid

#111077

Purpose

Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RaACR_687

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRB_p2_TCRA

Plasmid

#131034

Purpose

Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Inserts

TCRB (Tcrb Mouse)
TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPM_Z

Plasmid

#148126

Purpose

Mammalian Expression of HshnRNPM

Depositor

Elisa Izaurralde

Insert

HshnRNPM (HNRNPM Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations compared to the sequenceof t…

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS5

Plasmid

#104354

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

A4V

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MS2-HA-HsMex3B_AD

Plasmid

#148478

Purpose

Mammalian Expression of HsMex3B

Depositor

Elisa Izaurralde

Insert

HsMex3B (MEX3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LTK-V5/HIS

Plasmid

#200116

Purpose

Expression of human LTK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human LTK receptor tyrosine kinase (LTK Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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