-
Plasmid#154321PurposeMulti-cassette for SapTrapDepositorInsertGFP^SEC (LoxP)^AID*::3xFLAG
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1886
Plasmid#154333PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1822
Plasmid#154322PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::GFP
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-CMV-Cdc42 3'UTR mutant (position 577-599)
Plasmid#51391DepositorInsertNM_001039802.1 (CDC42 Human)
UseLuciferaseTagsLuciferaseExpressionMutationmiR-29 binding site mutant (position 577-599)PromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)
Plasmid#51390DepositorInsertNM_001039802.1 (CDC42 Human)
UseLuciferaseTagsLuciferaseExpressionMutationmiR-29 binding site mutant (position 577-599, 997…PromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp12
Plasmid#218286PurposeMulti-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modulesDepositorInsertHO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1
Plasmid#182944PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTRDepositorInsertMNTS-1 (NTF3 Synthetic, Homo sapiens, Human)
UseLentiviralTagsExpressionMammalianMutationThis is the Human NT-3 with mutations on the matu…PromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1816
Plasmid#154320PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1827
Plasmid#154323PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-IGK-dAPEX2-KDEL
Plasmid#117183PurposePlasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-IGK-dAPEX2-KDEL
Plasmid#117184PurposePlasmid name in publication: pAAV-FDIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAV; Flp/frtTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV SpCas9
Plasmid#206268PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertSpCas9
UseCRISPR; Recombinant baculovirus productionTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV EYFP Tubulin
Plasmid#206254PurposeENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.DepositorInsertEYFP Tubulin
UseMultimate/gateway entr 1TagsEYFPExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 CMV mTFP1 Actin
Plasmid#206255PurposeENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.DepositorInsertmTFP1 Actin
UseMultimate/gateway entr 2TagsmTFP1ExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 3 CMV mito mCherry
Plasmid#206256PurposeENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.DepositorInsertmito mCherry
UseMultimate/gateway entr 3TagsCOX8 mitochondrial tagExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV GST mTagBFP
Plasmid#206259PurposeENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.DepositorInsertGTS mTagBFP
UseMultimate/gateway entr 4TagsBFGT1 (Golgi localisation peptide)ExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV mAG beta-catenin
Plasmid#206258PurposeENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.DepositorInsertmAG Ctnnb1 (Ctnnb1 Mouse)
UseMultimate/gateway entr 1Tagsmonomeric Azami GreenExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Start-Stop Assembly Toolkit
Plasmid Kit#1000000152PurposeStart-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.DepositorAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
UbC-TRE-mSOD2-tTS-IRES-EGFP
Plasmid#12390DepositorInsertmSOD2 shRNA (Sod2 Mouse)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P2
Plasmid#194344PurposesgRNA position entry clone 2DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only