Addgene

We narrowed to 9,197 results for: hwa

Showing: 1981 - 2000 of 9197 results

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SeeSaw Reporter (SSR2.0)

Plasmid

#50393

Purpose

SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.

Depositor

Pablo Huertas Sánchez

Article

Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

Insert

SSR2.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

Plasmid

#84603

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

ODC1 destabilizing element

Expression

Mutation

Promoter

8xGliBS-delta-crystallin minimal promoter

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTUP1

Plasmid

#104341

Purpose

Express TUP1

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

TUP1 (TUP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pELuc

Plasmid

#118056

Purpose

GBPart - ELuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

ELuc

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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HA-sav

Plasmid

#32834

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Sav (SAV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
myc-mTOR (1-1482 aa)

Plasmid

#21745

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1-1482) (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

amino acids 1-1482

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
Omega 1

Plasmid

#118046

Purpose

GoldenBraid2.0 Omega 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMiniP

Plasmid

#118049

Purpose

GBPart - Minimal Promoter for expression in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

minimal TATA-box promoter with low basal activity

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242/287A

Plasmid

#32822

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242/287A

Promoter

CMV

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pCN-mBFP

Plasmid

#63847

Purpose

Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Insert

mBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter and RBS was swapped. His-tag was removed…

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SH3BP4

Plasmid

#46891

Purpose

expresses HA tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
Flag-NRF1-HA

Plasmid

#34708

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pET23-PBD(65-150)-N-Cys

Plasmid

#13722

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD(65-150)-N-Cys (PAK1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Met-Cys-Gly-PBD(65-150)-

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
pFLAG-ZYG11B

Plasmid

#110550

Purpose

expresses a FLAG-tagged human ZYG11B protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11B (ZYG11B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSUPER IKKe

Plasmid

#26211

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK epsilon (IKBKE Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pBI-p53 R175H/EGFP

Plasmid

#16544

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R175H

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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hNFAT2-409:TALE:VP64:KRɸ

Plasmid

#160661

Purpose

Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

Depositor

Roman Jerala

Article

Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

Insert

hNFAT2-409:TALE:VP64:KRɸ

Use

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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