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Plasmid#119148PurposeEncodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 5 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_38
Plasmid#59961PurposeHigh core fragment expression (high resource allocator)DepositorInsertcore fragment
UseTagsExpressionBacterialMutationN/APromoterPJ23105Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYCTK025
Plasmid#176729PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Lachancea mirantina (receiver part).DepositorArticleInsertste2Lm
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK028
Plasmid#176732PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).DepositorArticleInsertste2Tp
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVY-3 sfGFP-tag3
Plasmid#194618Purposefluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coliDepositorInsertsfGFP-K(GGSKKRKKR)3
UseTagsKGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGAExpressionBacterialMutationPromoterT7Available sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCTX-myc-HIPK2 KD
Plasmid#32997DepositorInsertHIPK2 (hipk2 Frog)
UseTags6XMycExpressionMutationK217>R (in the catalytic lysine) and STY348-35…PromoterSCMVAvailable sinceApril 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYCTK009
Plasmid#176713PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Eremothecium cymbalariae (sender part).DepositorArticleInsertmfalpha1Ec
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK020
Plasmid#176724PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Eremothecium cymbalariae (receiver part).DepositorArticleInsertste2Ec
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK030
Plasmid#176734PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Candida albicans (barrier part).DepositorArticleInsertbar1Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK031
Plasmid#176735PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Eremothecium cymbalariae (barrier part).DepositorArticleInsertbar1Ec
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK008
Plasmid#176712PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Candida albicans (sender part).DepositorArticleInsertmfalpha1Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_39
Plasmid#59962PurposeLow core fragment expression (low resource allocator)DepositorInsertcore fragment
UseTagsExpressionBacterialMutationN/APromoterPJ23105Available sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CII
Plasmid#51566PurposeExpression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcII
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJBM1
Plasmid#225288PurposeE. coli- C. coli shuttle vector; KanRDepositorInsertReplication elements from pWestmill 11
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorInsertplsB, plsC, cdsA, pssA
UseCell-free expressionTagsExpressionMutationPromoterT7 for each geneAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{PDK-198}:NosT
Plasmid#212176PurposeThis binary vector expresses mNeonGreen containing the catalase (PDK) intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PDK-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PDK int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVY-17 TC-TEM1-tag3
Plasmid#194631Purposeantibiotic resistance enzyme with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-TEM1-GGSKKRKKRGGSKKRKKRGGSKKRKKR
UseTagsGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterpBADAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-19 TC-sfGFP-tag3
Plasmid#194633Purposefluorescent protein with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-sfGFP-K(GGSKKRKKR)3
UseTagsKGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-11 mScarletI-tag3
Plasmid#194626Purposefluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coliDepositorInsertmScarlet-I-(GGSKKRKKR)3
UseTagsGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-10 mScarletI-tag2
Plasmid#194625Purposefluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coliDepositorInsertmScarlet-I-(GGSKKRKKR)2
UseTagsGGSKKRKKRGGSKKRKKR and MGHHHHHGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only