Addgene

We narrowed to 3,013 results for: actin

Showing: 1981 - 2000 of 3013 results

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pRK5-mWnt8a

Plasmid

#42284

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8a (Wnt8a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pNVLTv2

Plasmid

#169245

Purpose

Generic suicide vector for knockouts and integrations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDS296

Plasmid

#34985

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb1

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pDS191

Plasmid

#34978

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-ClbN-NHis

Plasmid

#51497

Purpose

NRPS module containing CATE domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbN

Use

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa30-1094 of NP_754347.1

Promoter

T7

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-clbB

Plasmid

#114155

Purpose

Expresses full-length ClbB

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbB

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-31 site

Plasmid

#25021

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-31 site

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pDS295

Plasmid

#34984

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH

Plasmid

#114156

Purpose

Expresses full-length ClbH

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTK729

Plasmid

#114710

Purpose

Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705+1700-2115ΔNLS)-RFP-Nano

Use

Tags

Expression

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPS934

Plasmid

#8855

Depositor

Pamela Silver

Article

Kahana et al Mol Biol Cell. 1998 Jul . 9(7):1741-56.

Insert

NUF2-sGFP (NUF2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
A0A0N1ND03

Plasmid

#163228

Purpose

Inducible expression of UniProt identifier A0A0N1ND03

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0N1ND03

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PAJ408

Plasmid

#104150

Purpose

dCas9 plasmid expressing full length mKate2 mRNA without RBS

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTK643

Plasmid

#114703

Purpose

Express NuMA(214-705)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(214-705)-RFP-Nano

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbP-S95A

Plasmid

#48245

Purpose

Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Use

Tags

Expression

Bacterial

Mutation

Changed Serine 95 to Alanine

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLG-Acc

Plasmid

#33148

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Use

Tags

LacZ

Expression

Yeast

Mutation

AccI site in intron was cut/filled/ligated to cha…

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pLG-Sty

Plasmid

#33149

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Use

Tags

LacZ

Expression

Yeast

Mutation

Sty1 site upstream of intron was cut/filled/ligat…

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pPS1701

Plasmid

#8852

Depositor

Pamela Silver

Article

Kahana et al Mol Biol Cell. 1998 Jul . 9(7):1741-56.

Insert

NIP100 (NIP100 Budding Yeast)

Use

Tags

3x HA

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 mycBioID

Plasmid

#35700

Purpose

To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA

Use

Tags

Myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

cmv

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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