Addgene

We narrowed to 3,036 results for: aaas

Showing: 1981 - 2000 of 3036 results

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pLJM60 FLAG-RAB35 S22N

Plasmid

#107106

Purpose

Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pmax pona 12xTRICK 24xMS2SL

Plasmid

#64542

Purpose

TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Use

Tags

24xMS2 stem loops, HA, NLS, and dNP799

Expression

Mammalian

Mutation

Promoter

ponA

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-shRab27a

Plasmid

#120930

Purpose

conditionally knockdown Rab27a in mouse cell lines

Depositor

Mien-Chie Hung

Article

Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

Insert

shRab27a (Rab27a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcw107-V5

Plasmid

#64649

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

Plasmid

#138295

Purpose

gRNA for CRISPR/Cas9 knockout of human TRIM21

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

gRNA TRIM21 (TRIM21 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000563266)

Plasmid

#80036

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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tet_pLKO.1_puro_shNRF2 #2

Plasmid

#136585

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v2 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shSTING-Mmu

Plasmid

#127697

Purpose

Doxycyclin inducible shRNA knockdown of mouse STING gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shSTING Mmu (Sting1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.U6.shRNA-Sdc1-1376

Plasmid

#195574

Purpose

Expresses shRNA to knockdown mouse Syndecan-1

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

shRNA, eGFP (Sdc1 Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN

Plasmid

#180647

Purpose

Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, has siRNA resistance to GAACAGUGUG…

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shRUNX1 puro

Plasmid

#45816

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

Runx1 (RUNX1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-CTCF-prom

Plasmid

#195104

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF promoter

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[SNCA]

Plasmid

#75437

Purpose

knockdown alpha-synuclein in rat neurons

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

snca (Snca Rat)

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-GFPg1 (BB09)

Plasmid

#139461

Purpose

Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

GFP-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Dnmt3b-R

Plasmid

#122333

Purpose

Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Dnmt3b

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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plKO.1-DNAJC5-ShRNA

Plasmid

#205729

Purpose

Knockdown of DNAJC5

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

shRNA targeting DNAJC5 (DNAJC5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJG367

Plasmid

#91185

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_H840A+gNt_R2+gNt_F2+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJG382

Plasmid

#91189

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gNt_R2+gNt_F2+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

Plasmid

#192683

Purpose

Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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