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pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163062

Purpose

Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Gateway entry vector

Tags

mOrange2

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52379

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4

Use

Tags

Expression

Mammalian

Mutation

Promoter

OCT4

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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BCR/ABL P190 transgenic construct

Plasmid

#38185

Depositor

Nora Heisterkamp

Article

Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

Insert

BCR/ABL P190 (ABL1, BCR Human)

Use

Construct for making transgenic mice

Tags

Expression

Mutation

Promoter

truncated mouse MT promoter

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pET co-transformation cloning vector (13S-A)

Plasmid

#48323

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
P-miR-145 (pTransluc-miR-145-1)

Plasmid

#21496

Depositor

Kenneth Kosik

Article

Xu et al Cell. 2009 May 15. 137(4):647-58.

Insert

1.5kb 5' of miR-145 (MIR145 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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p139 pETF-GST RanBP10 L301I

Plasmid

#11092

Depositor

Ramesh Shivdasani

Insert

RanBP10 (Ranbp10 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

L301I; see depositor comments below

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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(P79) OCN/Wnt 10b transgene

Plasmid

#25878

Depositor

Ormond MacDougald

Insert

Wnt10b (Wnt10b Mouse)

Use

Mouse Targeting

Tags

B-globin intron/poly A coding sequence and OCN pr…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only
p140 pETF-GST RanBP10 L301G

Plasmid

#11093

Depositor

Ramesh Shivdasani

Insert

RanBP10 (Ranbp10 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

L301G

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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p141 pETF-GST RanBP10 G308R

Plasmid

#11094

Depositor

Ramesh Shivdasani

Insert

RanBP10 (Ranbp10 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

G308R

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

Plasmid

#51656

Purpose

hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

Depositor

Adam Cohen

Insert

Quasar2-mOrange-Chr64-GFP

Use

Lentiviral

Tags

GFP and mOrange

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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delta DE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52381

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -3194 - -1768 upstream from OCT4 ATG

Promoter

OCT4

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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mouse deltaPE Oct4-GFP transgenic reporter

Plasmid

#52382

Purpose

BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP pA
Oct4 (Pou5f1 Mouse)
PGK-gb2-Neo

Use

Tags

Expression

Mammalian

Mutation

deleted -300 - -1300bp fragment upstream from Oct…

Promoter

Oct4 and PGK+gb2

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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mouse Oct4-GFP GOF18 transgenic reporter

Plasmid

#60527

Purpose

BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

GFPpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Oct4

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTRANS_253

Plasmid

#91125

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPSmOrange-N1

Plasmid

#31898

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pTRANS_220

Plasmid

#91113

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange

Plasmid

#177827

Purpose

Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPSmOrange2-N1

Plasmid

#34958

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Use

Tags

Expression

Mammalian

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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