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Showing: 181 - 200 of 646 results
  1. pCRISPR2_+10D+10U

    Plasmid
    #149394
    Purpose
    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site
    Depositor
    Insert
    Pseudomonas aeruginosa PA14 CRISPR2 locus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    10bp inserted upstream and downstream of the IHFp…
    Promoter
    pBAD
    Available since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pGP-AAV-GFAP-iGABASnFR2-WPRE

    Plasmid
    #218871
    Purpose
    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)
    Depositor
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102Y F104Y L178S
    Promoter
    GFAP
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid
    #218874
    Purpose
    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)
    Depositor
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102Y F104Y L178S
    Promoter
    Synapsin
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE

    Plasmid
    #218873
    Purpose
    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)
    Depositor
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2(no bind)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102G R168P
    Promoter
    GFAP
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pGP-AAV-syn-iGABASnFR2(no bind)-WPRE

    Plasmid
    #218876
    Purpose
    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)
    Depositor
    Has Service
    AAV1 and AAV5
    Insert
    iGABASnFR2(no bind)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102G R168P
    Promoter
    Synapsin
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pGP-AAV-CAG-flex-iGABASnFR2-WPRE

    Plasmid
    #218880
    Purpose
    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)
    Depositor
    Insert
    iGABASnFR2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    S99A F102Y F104Y L178S
    Promoter
    CAG
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pGP-AAV-syn-flex-iGABASnFR2n-WPRE

    Plasmid
    #218878
    Purpose
    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)
    Depositor
    Insert
    iGABASnFR2n
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    S99A F104H R168P
    Promoter
    Synapsin
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pGP-AAV-CAG-flex-iGABASnFR2n-WPRE

    Plasmid
    #218881
    Purpose
    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)
    Depositor
    Insert
    iGABASnFR2n
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    S99A F104H R168P
    Promoter
    CAG
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pCRISPR-mcBEST

    Plasmid
    #209416
    Purpose
    Plasmid for multiplexed cytosine base editing in streptomycetes
    Depositor
    Inserts
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    base editing cassette: PtipA ; sgRNAs: PkasO* an…
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pSG020

    Plasmid
    #126503
    Purpose
    Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHis
    Depositor
    Insert
    disaggregase ClpGgi with 6xHis-tag
    Use
    Tags
    C-terminal 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. pQE81L-KOFP7

    Plasmid
    #160330
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.
    Depositor
    Insert
    KOFP7
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pCas1_Cas2/3

    Plasmid
    #89240
    Purpose
    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1
    Depositor
    Insert
    Cas1 and Cas2/3
    Use
    Tags
    Strep II tag on Cas1
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 646 results