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Plasmid#24630DepositorInsertfadD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pVSV eGFP dG
Plasmid#31842DepositorInsertVSV genome
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
p6XHis-T7(P266L)
Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
UseTags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
OgeuIscB ωRNA-v13
Plasmid#219634Purposehuman U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloningDepositorInsertOgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_tdTomato
Plasmid#198041PurposeBxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.DepositorInserttdTomato
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVSV eGFP RABV-G
Plasmid#31833DepositorInsertVSV genome
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKB817 (pBra-Hfq)
Plasmid#174661PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.DepositorInsertHfq
UseTagsalpha NTDExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJin-278
Plasmid#125714PurposeABA-inducible split T7 GFP vector using RNAPN d5-19DepositorInsertPT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
UseTagsExpressionBacterialMutationSUMO tag fused to N-terminus of PylBPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEV_S
Plasmid#100266PurposepDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coliDepositorInsertsUseSynthetic Biology; Expression, pbr322TagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pReg-ROTU4
Plasmid#190669PurposeExpresses an evolved rotundine biosensor in E. coliDepositorInsertROTU4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-THP4
Plasmid#190671PurposeExpresses an evolved tetrahydropapaverine biosensor in E. coliDepositorInsertTHP4
UseSynthetic BiologyTagsExpressionMutationPromoterJ23103Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGfOMT1-GEN4
Plasmid#190612PurposeExpresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coliDepositorInsertGfOMT-GEN4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-GLAU4
Plasmid#190667PurposeExpresses an evolved glaucine biosensor in E. coliDepositorInsertGLAU4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-PAP4
Plasmid#190668PurposeExpresses an evolved papaverine biosensor in E. coliDepositorInsertPAP4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-NOS4
Plasmid#190670PurposeExpresses an evolved noscapine biosensor in E. coliDepositorInsertNOS4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-RTA
Plasmid#99675PurposeExpresses dSp Cas9 fused at C term. To VP64 and RTA activation domainsDepositorArticleInsertdCas9
UseTagsVP64-RTAExpressionMammalianMutationdead Cas9PromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH577
Plasmid#172161PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylD…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only