Skip to main content
Addgene
Showing: 181 - 200 of 553 results

  1. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

    Plasmid
    #122959
    Purpose
    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xmyc tag
    Expression
    Mutation
    deletion of C terminus from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122959-plasmid-map-sequence-id-244512

    Enlarge

  2. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

    Plasmid
    #122956
    Purpose
    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xhis tag
    Expression
    Mutation
    deletion of C terminus from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122956-plasmid-map-sequence-id-244423

    Enlarge

  3. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid
    #122955
    Purpose
    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xhis tag
    Expression
    Mutation
    deletion of EGF repeats from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122955-plasmid-map-sequence-id-244415

    Enlarge

  6. pMA3790

    Plasmid
    #70110
    Purpose
    mtDNA elimination/production of rho-0 cells
    Depositor
    Mikhail Alexeyev
    Article
    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi
    Inserts
    EGFP
    hUNG1 Y147A
    Neo
    Use
    Tags
    None, human OTC mitochondrial targeting sequence,…
    Expression
    Mammalian
    Mutation
    Y147A, aa's 1-77 removed
    Promoter
    CMV, EF1a, and SV40 and native
    Available since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    70110-plasmid-map-sequence-id-399654

    Enlarge

  8. CMV/N3IC-HA

    Plasmid
    #47618
    Purpose
    in vitro transcription and translation of HA-tagged Notch3 Intracellular domain
    Depositor
    Urban Lendahl
    Article
    Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.
    Insert
    Notch3 Intracellular domain (Notch3 Mouse)
    Use
    In vitro transcription and translation
    Tags
    HA
    Expression
    Mammalian
    Mutation
    codons 1664 (Met) to 2318 (Ala)
    Promoter
    CMV
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pHR_PGK_antiCD19_eZ3_Gal4VP64

    Plasmid
    #169917
    Purpose
    Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.
    Depositor
    Zhili Rong
    Article
    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do
    Insert
    antiCD19-Notch3-GL4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Additional EGF repeat between the extracellular d…
    Promoter
    PGK
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169917-plasmid-map-sequence-id-336047

    Enlarge

  14. pWZL-N1ICD-Flag

    Plasmid
    #171171
    Purpose
    Retroviral vector for expression of NOTCH1 intracellular domain with a C-terminal FLAG tag (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    NOTCH1 intracellular domain (NOTCH1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Will express residues 1758-2556 of human NOTCH1, …
    Promoter
    Viral LTR
    Available since
    Availability
    Academic Institutions and Nonprofits only
    171171-plasmid-map-sequence-id-338649

    Enlarge

  16. pLIX-hN1ICD

    Plasmid
    #91897
    Purpose
    Doxycycline inducible expression of human Notch1 ICD
    Depositor
    Julien Sage
    Insert
    Notch1 intracellular domain (NOTCH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codons 1770 to 2555 of human NOTCH1
    Promoter
    TRE promoter, Tet ON
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91897-plasmid-map-sequence-id-180657

    Enlarge

Showing: 181 - 200 of 553 results