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Plasmid#201771PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c46
Plasmid#201774PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c25
Plasmid#201769PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterCYC1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c15
Plasmid#201768PurposeExpresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterADH1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAl-pelB-DB21
Plasmid#226994PurposeExpress nanobody DB21 in E.coliDepositorInsertDB21
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAl-pelB-CA14
Plasmid#226993PurposeExpress nanobody CA14 in E.coliDepositorInsertCA14
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP434
Plasmid#104167PurposeBacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
UseTags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation3x Cysteines located at bps 493-495, 520-522, and…PromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-1xTS
Plasmid#109418PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
hIgG1_heavy_CoVMab7
Plasmid#194605PurposeMammalian expression of CoVMab7 heavy chainDepositorInsertCoVMab7 heavy chain (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1_heavy_CoVMab4
Plasmid#194603PurposeMammalian expression of CoVMab4 heavy chainDepositorInsertCoVMab4 heavy chain (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1_kappa_CoVMab7
Plasmid#194606PurposeMammalian expression of CoVMab7 light chainDepositorInsertCoVMab7 light chain (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1_kappa_CoVMab4
Plasmid#194604PurposeMammalian expression of CoVMab4 light chainDepositorInsertCoVMab4 light chain (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csGBP
Plasmid#108874PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFPDepositorInsertAPEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csChBP
Plasmid#108876PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherryDepositorInsertAPEX2-csChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
APEX2-ChBP
Plasmid#108878PurposeExpression of APEX2 fused to a nanobody against mCherryDepositorInsertAPEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMito-GBPen
Plasmid#128269PurposeExpression of mitochondrially targeted GBPen (GFP Nanobody)DepositorInsertMito-GBPen
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-ChBP
Plasmid#108892PurposeMultisite gateway vector for 3' tagging with a nanobody against mCherryDepositorInsertChBP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceJune 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
MARS-nGFP
Plasmid#205234PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
UseTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-csGBP
Plasmid#108875PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)DepositorInsertmKate2-P2A-APEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only